MEK/ERK信号通路对人胶质母细胞瘤U87细胞中转录因子Nrf2核积累的影响  

Effect of the MEK/ERK signaling pathway on nuclear accumulation of Nrf2 in U87 human glioblastoma cell line

作　　者：丛子翔[1] 王汉东[1] 王嘉炜[1] 潘灏[1] 周渊[1] 

机构地区：[1]南京大学医学院临床学院南京军区南京总医院,南京210093

出　　处：《山东医药》2013年第13期14-16,共3页Shandong Medical Journal

基　　金：江苏省医学重点学科基金资助项目(X4200722)

摘　　要：目的探讨在人胶质母细胞瘤U87细胞中MEK/ERK信号通路对转录因子NF-E2相关因子2(Nrf2)核积累的影响。方法用MEK/ERK信号通路特异性抑制剂PD98059处理U87细胞2 h后,免疫荧光染色法评估其对Nrf2蛋白分布的影响,用Western blot法分析核内Nrf2蛋白水平的变化。结果 MEK/ERK信号通路抑制剂PD98059能抑制Nrf2的核转移,显著降低核内Nrf2的蛋白水平。结论人胶质母细胞瘤U87细胞中异常激活的ERK信号通路与转录因子Nrf2的核积累密切相关。Objective To study the effect of the MEK/ERK signaling pathway on nuclear accumulation of Nrf2 in U87 human glioblastoma cell line. Methods U87 human glioblastoma cells were treated for 2 hours with the MEK/ERK path- way inhibitor PD98059, and the effects on the distribution of Nrf2 were evaluated by immunofluorescence. The protein level of nuclear Nrf2 was analyzed by Western blot. Results The MEK/ERK pathway inhibitor PD98059 suppressed the nuclear translocation of Nrf2 and reduced the protein level of nuclear Nrf2 significantly. Conclusion Nuclear accumulation of Nrf2 is closely related with the aberrant activation of the MEK/ERK pathway in U87 human glioblastoma cell line.

关 键 词：胶质母细胞瘤 MEK ERK信号通路 转录因子NF—E2相关因子2 

分 类 号：R739.41[医药卫生—肿瘤]