纤维素酶曲的浅盘生产研究  

Studies on the Conditions for Cellulase Production from A Trichoderma reesei Strain

作　　者：张加春[1] 易琴[1] 黄遵锡[1] 

机构地区：[1]云南师范大学生命科学系,云南昆明650092

出　　处：《云南师范大学学报（自然科学版）》2000年第2期50-52,共3页Journal of Yunnan Normal University:Natural Sciences Edition

基　　金：云南省科技攻关资助项目

摘　　要：本试验以里氏木霉 Tr-H为出发菌株 ,对其浅盘培养条件及酶活测定方法进行优化 ,结果 ,当接种量为 0 .5% ,初始 p H值 2 .0 ,料层厚度 2 .5cm时 ,2 8℃培养 1 60小时 ,50℃浸提 ,酶活从原来的2 80 mg G/g.h提高到 60 0 mg G/g.h;扣盘使酶活下降。A Trichoderma reesei strain Tr-H with high potency for cellulase production was studied in this paper. The results showed that the best conditions for producing cellulase were: inoculating quantity 0.5%, original pH2.0, material thickness 2.5 cm, culture temperature 28℃, extraction temperature 50℃, cultured at that condition for 160 hours,the filter paper enzyme activity raised from 280mgG/g.h to 600mgG/g.h.

关 键 词：里氏木霉 纤维素酶 酶活 浅盘生产 

分 类 号：Q539.3[生物学—生物化学] Q55