粘质沙雷氏菌G1利用玉米浆干粉和磷酸氢二铵生产2,3-丁二醇的研究  

Study on 2,3-Butanediol Production by Serratia Marcescens G1 Using Corn Steep Liquor Powder and Ammonium Dibasic Phosphate

作　　者：郝英利[1] 张燎原[1] 孙建安[1] 魏东芝[1] 周文瑜[1] 沈亚领[1] 朱家文[2] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室鲁华生物技术研究所,上海200237 [2]华东理工大学化工分离研究所,上海200237

出　　处：《化学与生物工程》2013年第6期34-38,共5页Chemistry & Bioengineering

基　　金：国家863计划资助项目(2006AA02Z243);国家重点实验室专项经费资助项目(2060204);上海市重点学科建设资助项目(B505)

摘　　要：研究了几种工业氮源对粘质沙雷氏菌G1发酵生产2,3-丁二醇的影响,在确定玉米浆干粉为氮源的基础上,利用Plackett-Burman(PB)实验和响应面法(RSM)实验对玉米浆干粉和磷酸氢二铵[(NH4)2HPO4]的浓度进行了优化,确定优化培养基(g·L-1)为:蔗糖90,玉米浆干粉20.32,(NH4)2HPO47.21,NaAc 4,柠檬酸钠14,MgSO40.5,Fe-SO40.02,MnSO40.01。并以此优化培养基进行了摇瓶和分批补料发酵,结果表明,摇瓶发酵中,90g·L-1的蔗糖最终被转化成43.06g·L-1的2,3-丁二醇;分批补料发酵中,2,3-丁二醇浓度为128.28g·L-1,产率为2.67g·L-1·h-1,转化率为0.48g·g-1蔗糖。以玉米浆干粉和(NH4)2HPO4为氮源,2,3-丁二醇浓度较高,培养基的成本大幅降低,为工业化生产奠定了基础。Effects of several industrial nitrogen sources on biomass and 2,3-butanediol production for Serra- tiarnarcescens G1 were studied. The results showed that corn steep liquor powder（CSLP） was the optimum in- dustrial nitrogen source. Based on the previous experiments, the optimum concentrations of CSLP and ammoni- um dibasic phosphate[（NH4）2HPO4] were optimized by PB design and response surface methodology（RSM）. The optimal medium was determined as follows： sucrose 90 g·L^-1, CSLP 20.32 g ·L^-1, （NH4） 2 HPO4 7.21 g·L^-1,NaAc 4 g·L^-1,sodium citrate 14 g·L^-1,MgSO4 0. 5 g ·L^-1,FeSO4 0.02 g ·L^-1,MnSO4 0. 01 g·L^-1. The optimal medium was used to perform shaking fermentation and fed-batch fermentation experiments. Ultimately,the strain converted 90 g·L^-1 sucrose to 43.06 g·L^-1 2,3-butanediol in shaking flasks, in fed- batch fermentation,a 2,3-butanediol level of 128.28 g·L^-1 Was obtained,and the transformation rate and yield of 2,3-butanediol achieved to 0.48 g·g^-1 and 2.67 g ·L^-1·h^-1 , respectively. It provides the foundation for industrial production that using CSLP and （NH4）2 HPO4 as nitrogen sources with higher production of 2,3-bu- tanediol,lower cost of medium.

关 键 词：粘质沙雷氏菌G1 玉米浆干粉 磷酸氢二铵 2 3-丁二醇 响应面法 成本 分批补料发酵 

分 类 号：TQ923[轻工技术与工程—发酵工程]