胃蛋白酶催化壳聚糖降解的特性研究  

STUDIES ON THE HYDROLYSIS OF CHITOSAN BY PORCINE PEPSIN

作　　者：陈云[1] 胡健[1] 赵国骏[1] 姜涌明[1] 

机构地区：[1]扬州大学生物科学与技术学院酶工程研究室,江苏扬州225009

出　　处：《扬州大学学报（自然科学版）》2000年第3期31-34,共4页Journal of Yangzhou University：Natural Science Edition

基　　金：江苏省"九五"重点攻关项目 !(BE96 433);江苏省教委科研基金项目!(D9778)

摘　　要：采用粘度测定法和还原糖测定法 ,研究了温度、p H值、反应时间、底物浓度、金属离子及壳聚糖的脱乙酰化度对胃蛋白酶降解活性的影响 ,确定了以壳聚糖为底物的猪胃蛋白酶的一些催化特性 :最适温度为 70℃ ,最适 p H值为 5.0 ,米氏常数 Km为 8.94× 1 0 -3 g· m L-1,一定浓度的 Ca2 + ,Cu2 + ,Ba2 + ,Mn2 +抑制该酶的活性 .结果表明 :随着壳聚糖脱乙酰化度的提高 ,胃蛋白酶活性降低 .The effects of reaction temperature, pH, substrate and enzyme concentration, some metal ions and the deacetylation degree of chitosan on the hydrolytic velocity of chitosan by porcine pepsin were investigated. The viscosity of chitosan solution was decreased quickly at the catalysis of pepsin. The optimum temperature and optimum pH were 70 ℃ and 5.0 respectively, and the K m value for chitosan was 8.94×10 -3 g·mL -1 . Ca 2+ , Mn 2+ , Cu 2+ , Ba 2+ were inhabitors, while ICH 2COOH activated the acti vity of pepsin. The results showed that the activity of pepsin was reduced while the degree of deacetylation of chitosan raised.

关 键 词：壳聚糖 胃蛋白酶 降解 

分 类 号：Q55[生物学—生物化学] TQ464.8[化学工程—制药化工]