趋化因子CXCL14在骨肉瘤中的表达及其与预后的关系  

Expression of chemokine CXCL14 in primary osteosarcoma and its association with prognosis

作　　者：卢金昌[1] 王晋[1] 雍碧城[1] 宋国徽[1] 赵志强[1] 唐清连[1] 邹昌业[1] 尹军强[1] 谢显彪[1] 沈靖南[1] 

机构地区：[1]中山大学附属第一医院骨肿瘤科,广东广州510080

出　　处：《南方医科大学学报》2013年第6期798-803,共6页Journal of Southern Medical University

基　　金：国家自然科学基金(81272940,81072193)~~

摘　　要：目的研究趋化因子CXCL14在骨肉瘤细胞和组织中的表达及其与患者预后的关系。方法利用RT-PCR、酶联免疫吸附法(ELISA)和荧光定量PCR技术检测4种骨肉瘤细胞株及40对骨肉瘤及癌旁肌肉中CXCL14的表达,CCK8细胞增殖实验、克隆形成实验观察CXCL14对U2OS细胞增殖的影响,免疫组化检测CXCL14在骨肉瘤组织中的表达,并运用Kaplan-Meier法进行生存分析。结果 CXCL14在骨肉瘤细胞株中的表达均高于正常成骨细胞,而且其在骨肉瘤组织的表达也显著高于癌旁肌肉组织(P<0.01)。干扰CXCL14表达能抑制U2OS的生长(P<0.05),经两条CXCL14干扰RNA处理后U2OS克隆形成率分别为(4.93±0.25)%和(5.03±0.0.31)%,均显著低于阴性对照组(8.03±1.16)%,差异有统计学意义(P<0.05)。Kaplan-Meier生存分析显示,CXCL14高表达的患者预后比低表达组差(P=0.02)。结论 CXCL14在骨肉瘤中高表达,它能促进骨肉瘤细胞的增殖并与患者不良预后相关,提示其可能成为潜在的治疗靶点。Objective To detect the expression of CXCL14 in human osteosarcoma cell lines and tissues and investigate its association with the prognosis of the patients. Methods RT-PCR, enzyme-linked immunosorbnent assay （ELISA） and real-time PCR were used to detect the expression of CXCL14 in 4 osteosarcoma cell lines and in 40 pairs of osteosarcoma tissues and adjacent muscular tissues. CCK8 assay and colony formation assay was used to assess the effect of CXCL14 suppression mediated by two specific siRNAs on the proliferation of U2OS osteosarcoma cells. Immunohistochemistry was performed to detect the expression of CXCL14 in 58 osteosarcoma tissues, and Kaplan-Meier method and log-rank test were performed for survival analysis of the patients. Results Significant up-regulation of CXCL14 expression was found in the osteosarcoma cell lines and in osteosarcoma tissues compared with the adjacent muscles （P〈0.01）. In U2OS cell, suppression of CXCL14 expression by siRNA significantly inhibited the cell proliferation （P〈0.01） and colony formation rate （P〈0.05）. Kaplan-Meier survival analysis indicated that patients with high CXCL14 expression had worse prognosis than those with low CXCL14 expression （P--0.02）. Conclusion CXCL14 is up-regulated in both osteosarcoma cell lines and primary osteosarcoma tissues to promote the proliferation of osteosarcoma cells. A high CXCL14 expression in osteosarcoma tissues is associated with a poor prognosis, suggesting the that CXCL14 serve as a potential therapeutic target for osteosarcoma.

关 键 词：CXCL14 骨肉瘤 趋化因子 

分 类 号：R738.1[医药卫生—肿瘤]