人结肠癌细胞Dickkopf-1的表达水平及意义  

Expression of Dickkopf-1 in human colon carcinoma cell lines

作　　者：于倩[1,2] 杨磊[1,2] 丁彦青[1,2] 赵震[1,2] 刘会平[1,2] 

机构地区：[1]南方医科大学南方医院病理科 [2]南方医科大学基础医学院病理学系//广东省分子肿瘤病理学重点实验室,广东广州510515

出　　处：《南方医科大学学报》2013年第6期923-925,933,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(K1010125)~~

摘　　要：目的检测六株结肠癌细胞中Dickkopf-1(DKK1)的表达并探讨其意义。方法在6株结肠癌细胞(SW480、SW620、HT29、LS174T、LOVO、HCT116)分别应用qPCR方法检测DKK1的mRNA表达水平。用Western blot方法和细胞免疫组化方法检测DKK1的蛋白表达情况。结果荧光定量分析显示,DKK1在HT29、HCT116中的表达高于其他四株细胞(P<0.05);Western blot显示,DKK1在HCT116、HT29中的表达同样高于其他四株细胞;细胞免疫组化染色显示,DKK1在六株结肠癌细胞株中呈阳性表达。结论 DKK1在6株人结肠癌细胞系中的表达具有差异性。Objective To investigate the expression of Dickkopf-l（DKK1） in 6 different colon carcinoma cell lines. Methods The expression of DKK1 protein were detected in SW480, SW620, HT29, LS174T, LOVO, and HCT116 cells lines using Western blot analysis and immunocytochemistry and the mRNA levels of DKK1 was detected using quantitative real-time PCR （qPCR）. Results The results of qPCR showed significantly higher expressions of DKK1 mRNA in HT29 and HCT116 cells than in the other 4 cells （P〈0.05）. Western blotting demonstrated that DKK1 was over-expressed in HCT116 and HT29 cells compared with that in the other cell lines. All the 6 cell lines were found to have positive DKKI expression by immunocytochemical staining. Conclusion DKKI shows differential expression patterns among the 6 human colon carcinoma cell lines.

关 键 词：人结肠癌细胞株 DICKKOPF-1 时实定量PCR Western BLOTTING 细胞免疫组织化学 

分 类 号：R735.35[医药卫生—肿瘤]