甘露糖敏感型绿脓杆菌制剂对肝癌细胞周期的作用机制研究  

The effects and mechanism of the pseudomonas aeruginosa mannose sensitive haemagglutination vaccine on the cell cycle of hepatocellular carcinoma

作　　者：郭忠义[1] 董兆如[1] 智绪亭[1] 王程浩[1] 陈泽婷[1] 李小玮[1] 姜志超[1] 王翔宇[1] 陈志强[1] 李涛[1] 

机构地区：[1]山东大学齐鲁医院普外科,济南250012

出　　处：《中华肝胆外科杂志》2013年第6期452-455,共4页Chinese Journal of Hepatobiliary Surgery

基　　金：国家自然科学基金（30901444）;山东大学创新基金（2010TS030）

摘　　要：目的探讨甘露糖敏感性绿脓杆菌制剂（pseudomonasaeruginosa mannosese nsitive haemagglutination vaccine，PA-MSHA）对肝细胞癌（hepatocellular carcinoma，HCC）细胞周期的作用及其机制。方法培养人肝癌细胞株MHCC97L及HepG2，以不同剂量的PA-MSHA作用于肝癌细胞。MTT实验检测PA-MSHA对细胞增殖的影响。流式细胞技术检测PA-MSHA对细胞周期的作用。Western blot检测PA-MSHA作用前后细胞周期蛋白CyclinD1、细胞周期蛋白依赖性激酶2（CDK2）、增殖细胞核抗原（proliferating cell nuclear antigen，PCNA）以及细胞周期蛋白激酶抑制剂p21和p27的表达情况。结果与对照组相比，PA-MSHA显著抑制MHCC97L和HepG2细胞的增殖，其作用呈剂量及时问依赖性（P〈0．05）。PA-MSHA显著诱导肝癌细胞周期阻滞，PA-MSHA作用后GOG1期和G2-M期细胞比例显著增高，而S期细胞比例则显著下降（P〈0．05）。PA-MSHA显著抑制CyclinD1、CDK2、PCNA蛋白的表达，而促进p21和p27的表达。外源性甘露糖可显著抑制PA-MSHA对肝癌细胞增殖和周期的作用（P〈0．05）。结论PA-MSHA通过调控细胞周期相关蛋白来诱导HCC细胞周期阻滞，抑制细胞增殖。这一作用是通过肝癌细胞表面的甘露糖的介导实现的。Objective To investigate the effects and mechanism of the pseudomonas aeruginosa mannose sensitive haemagglutination vaccine （PA-MSHA） on the cell cycle of hepatocellular carcinoma （HCC）. Methods MHCC97L and HepG2 cells were cultured and treated with different doses of PAMSHA and mannose. MTT and flow cytometry were used to measure cell proliferation and cell cycles, respectively. The expressions of cell cycle related proteins Cyclin D1, CDK2, PCNA, p21, and p27 were evaluated by Western blot. Results Compared with the control group, the proliferation of HCC Cells in the PA-MSHA treated group was significantly inhibited in a time and dose dependant manner （P〈0.05）. PA-MSHA significantly arrested HCC cells in the G0-G1 and G2-M phase of the cell cycle, thereby decreasing the proportion of cells in the S phase （P〈0.05）. Western blot analysis revealed that PA-MSHA significantly inhibited the expressions of Cyclin D1, CDK2, PCNA, and significantly promoted the expression of p21 and p27. Preincubation of PA-MSHA with mannose significantly inhibited the effects of PA-MSHA on proliferation and cell cycle （P〈0.05）. Conclusion By regulating the expression of cell cycle related proteins, PA-MSHA significantly induced HCC cell cycle arrest and inhibited cell proliferation. These effects were mediated through PA-MSHA recognizing and combining to the mannose of HCC cells.

关 键 词：癌 肝细胞 绿脓杆菌制剂 甘露糖 增殖 

分 类 号：R735.7[医药卫生—肿瘤]