血管内皮生长因子C和nm23-H1蛋白在Ⅱ期和Ⅲ期结直肠癌组织中的表达及意义  

Expression and clinical significance of vascular endothelial growth factor-C and nm23-H1 in stage II and III colorectal carcinomas

作　　者：耿倩倩[1] 李毅[1] 唐春卉[3] 李恩孝[1] 吴胤瑛[1] 张冠军[2] 

机构地区：[1]西安交通大学医学院第一附属医院肿瘤内科,710061 [2]西安交通大学医学院第一附属医院病理科,710061 [3]陕西省人民医院老年内科

出　　处：《中华肿瘤杂志》2013年第6期439-444,共6页Chinese Journal of Oncology

摘　　要：目的探讨血管内皮生长因子c（VEGF—c）和nm23-H1蛋白在Ⅱ期和Ⅲ期结直肠癌组织中的表达水平及其临床意义。方法采用免疫组化法检测110例Ⅱ期和Ⅲ期结直肠癌患者肿瘤组织中VEGF-C和nm23-H1蛋白的表达水平，以53例癌旁组织作为对照；分析VEGF—C和nm23．H1蛋白的表达与Ⅱ、Ⅲ期结直肠癌患者临床病理特征及预后的关系。结果VEGF．C蛋白在结直肠癌组织中的阳性表达率为71．8％，明显高于癌旁正常组织（22．6％，P〈0．001）。nm23-H1蛋白在结直肠癌组织中的阳性表达率57．3％，明显低于癌旁正常组织（90．6％，P〈0．001）。VEGF．C蛋白在结直肠癌组织中的表达与淋巴结转移有关（P〈0．001），nm23-H1蛋白在结直肠癌组织中的表达与病理学类型和淋巴结转移有关（均P〈0．05）。VEGF-C和nm23-H1蛋白的表达与结直肠癌患者的性别、年龄、肿瘤原发部位、肿瘤大小和浸润深度均无关（均P〉0．05）。在结直肠癌组织中，VEGF-C蛋白与nm23-H1蛋白的表达呈负相关（r=-0．361，P〈0．001）。110例结直肠癌患者的中位生存时间为55个月，中位无病生存时间为48个月。VEGF-C和nm23-H1蛋白不同表达状态结直肠癌患者的5年总生存率和5年无病生存率的差异均有统计学意义（均P〈0．001），VEGF-C蛋白阴性/nm23-H1蛋白阳性患者的预后较好。结论VEGF—C和nm23-H1蛋白表达水平的联合检测对预测Ⅱ、Ⅲ期结直肠癌患者的预后具有重要的临床意义，但是否可作为判断结直肠癌患者预后的标志物仍需进-步研究。Objective To discuss the expression and clinical significance of VEGF-C and nm23-H1 in stage II and III colorectal carcinomas. Methods SP immunohistochemical staining was employed to determine the expression of vascular endothelial growth factor-C （VEGF-C） and nm23-H1 in the tumor tissues of 110 cases of stage 1I and HI colorectal carcinomas and in the adjacent mucosal tissues of 53 cases as control, and analyze their correlation with cliniopathological features and prognosis. Results The positive expression of VEGF-C in the carcinoma tissues was 71.8%, significantly higher than that in the adjacent mucosal tissues （22.6%, P 〈 0. 001 ）. The positive expression of nm23-H1 in the carcinoma tissues was 57.3%, significantly lower than that in the adjacent mucosal tissues （90.6%, P 〈 0.001 ）. The expression of VEGF-C was significantly correlated with lymph node metastasis （P 〈 0.05 ）, and the nm23- HI expression was significantly correlated with lymph node metastasis and pathological type （ P 〈 0.05 ）. The expression of VEGF-C and nm23-H1 did not show a significant correlation with age, gender, primary tumor site, tumor size and depth of invasion （ P 〉 0.05 ）. The VEGF-C expression was negatively related with nm23-H1 expression in colorectal carcinoma （ r = - 0. 361, P 〈 0.001 ）. The median overall survival （MOS） and median disease free survival （MDFS） of 110 patients with colorectal carcinoma were 55 and 48 months, respectively. The colorectal patients with different VEGF-C and nm23-H1 expression showed significant differences in the 5-year OS rate and 5-year DFS rate （P 〈 0.001 ）. The patients with negative VEGF-C expression and positive nn^3-H1 expression had a better prognosis. Conclusions The joint detection of VEGF-C and nm23-H1 expression is very promising in prediction of the prognosis of patients with stage II and III colorectal carcinoma. However, whether it can be used as a marker in prognosis judgment needs further investigation.

关 键 词：结直肠肿瘤 血管内皮生长因子C NM23-H1 免疫组织化学 预后 

分 类 号：R735[医药卫生—肿瘤]