丰富环境和贫瘠环境对锰中毒小鼠学习记忆能力的影响  

Effects of enriched environment and impoverished environment on learning and memory ability of manganese-exposed mice

作　　者：郭忠信[1] 李文宇 李骏然 李洪霖 韦轲 杨伯宁[1] 

机构地区：[1]广西医科大学人体解剖学教研室,南宁530021 [2]基础医学院

出　　处：《中华劳动卫生职业病杂志》2013年第6期409-412,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：广西自然科学基金资助（2010GXNSFA013157）

摘　　要：目的探讨丰富环境与贫瘠环境对锰染毒小鼠学习记忆能力的影响及其作用机制。方法将40只雌性昆明小鼠随机分为对照组、正常环境染锰组（模型组）、丰富环境染锰组（丰富组）以及贫瘠环境染锰组（贫瘠组），每组10只。采用腹腔注射氯化锰的方式制造小鼠锰中毒模型，Morris水迷宫试验检测小鼠学习和记忆能力，用免疫组织化学法检测小鼠海马区CREB蛋白的表达。结果在水迷宫定位航行试验中，模型组逃避潜伏期明显高于对照组，丰富组的逃避潜伏期低于模型组，差异有统计学意义（P〈0．05），贫瘠组与模型组的差异无统计学意义（P＞0．05）。空间探索实验中，丰富组的穿越平台次数则比模型组多，贫瘠组比模型组少，差异有统计学意义（P〈0．05）。模型组和贫瘠组海马CAl区CREB蛋白表达量明显低于对照组，差异有统计学意义（P〈0．05）；丰富组海马CA1区CREB蛋白表达量明显比模型组高，差异有统计学意义（P〈0．05）。结论丰富环境干预可改善锰中毒小鼠的学习记忆能力，增加海马内CREB的表达；丰富环境干预改善锰中毒小鼠学习记忆能力的机制可能与CREB的表达有关。Objective To investigate the effects of enriched environment and impoverished environment on the learning and memory ability of manganese-exposed mice and the mechanism. Methods Forty fe- male Kunming mice were randomly and equally divided into 4 group： control group （CG）, standard environment and manganese exposure group （SEG）, enriched environment and manganese exposure group （EEG）, and im- poverished environment and manganese exposure group （IEG）. The mouse model of manganese poisoning was established by intraperitoneal injection of manganese chloride. The learning and memory ability was tested by Morris water maze. The expression of cAMP response element-binding protein （CREB） in area CA1 of the hippocampus was measured by immunohistochemistry. Results In place navigation test, the SEG had a significantly longer escape latency than the CG （P〈0.05）, and the EEG had a significantly shorter escape latency than the SEG （P〈0.05）; there was no significant difference in escape latency between lEG and SEG （P〉0.05）. In spatial probe test, the EEG had a significantly greater number of platform crossings than the SEG （P〈0.05）, and the IEG had a significantly smaller number of platform crossings than the SEG （P〈0.05）. The expression of CREB in area CA1 of the hippocampus was significantly lower in IEG and SEG than in CG （P〈0.05）, and it was significantly higher in EEG than in SEG （P〈0.05）. Conclusion In the enriched environment, the learning and memory ability of manganese-exposed mice can be improved, which may be due to the increased expression of CREB in the hippocampus.

关 键 词：丰富环境 贫瘠环境 CREB 锰中毒 学习记忆 

分 类 号：R363[医药卫生—病理学]