顺铂对人胃癌细胞系p53β表达的影响及其生物学意义  

The expression of p53β and its biological significance induced by cisplatin on human gastric cancer cell line

作　　者：贾飞飞[1] 季万胜[2] 曹玉[1] 李蕾[2] 翟晴晴[1] 高志星[2] 

机构地区：[1]潍坊医学院研究生部,山东潍坊261041 [2]潍坊医学院附属医院消化内科

出　　处：《中华消化病与影像杂志（电子版）》2012年第6期33-37,共5页Chinese Journal of Digestion and Medical Imageology(Electronic Edition)

摘　　要：目的研究顺铂对野生型p53人胃癌细胞系MKN45 p53β表达的影响,并探讨其生物学意义。方法将不同浓度顺铂(2、4、6 mg/L)作用于野生型p53人胃癌细胞系MKN45。采用MTT法检测胃癌细胞增殖抑制率;RT-PCR法测定p53β、p53、Bax mRNA表达。不同浓度顺铂作用不同时间后胃癌细胞增殖抑制率比较、不同浓度顺铂作用后胃癌细胞p53β、p53、Bax mRNA的相对表达量比较均应用one way ANOVA方差分析,进一步组间两两比较均应用LSD-t检验;p53βmRNA与野生型p53 mRNA、Bax mRNA表达相关性分析应用Pearson直线相关分析。结果 MTT结果显示,随着顺铂浓度增加,作用时间的延长,细胞抑制率均逐渐增加,且组间差异均有统计学意义。RT-PCR结果显示,随着顺铂浓度增加,MKN45细胞中p53β、p53及Bax mRNA表达均逐渐增加,且组间差异均有统计学意义。Pearson直线相关分析结果显示,p53β与野生型p53及Bax表达呈正相关(r=0.976,P<0.05;r=0.985,P<0.05)。结论在顺铂诱导MKN45胃癌细胞增殖抑制中,p53β异构体表现出与野生型p53及其下游分子Bax协同作用,推测p53β异构体在胃癌细胞生长抑制效应中起着重要作用。Objective To explore the expression of p53β and its biological significance induced by eisplatin in human gastric cancer cell line MKN45 （wild-type p53 ）. Methods The different concentrations of eisplatin （ 2-4,6 mg/L ） effeeted on the wild type p53 in human gastric cancer cell line MKN45. The inhibition rate of proliferation were detected in gastric cancer cells by MTT assay; The expression of p53β, p53 and Bax mRNA were detected by using reverse transcription-polymerase chain reaction （RT-PCR）. Gastric cancer cell proliferation inhibition rate comparison of different concentrations of cisplatin in different time, the p53β,p53, Bax mRNA relative expression level comparison of different concentrations of eisplatin effected on gastric cancer cell, the two comparisons both used one way ANOVA variance analysis; the comparisons between any two groups were used LSD-t test; the correlation analysis of the expression of p53β mRNA and wild type p53 mRNA,Bax mRNA were used Pearson correlation analysis. Results MTT results showed that, with the increasing concentrations of cisplatin and the extension of treatment time, the inhibitory rate of cell increased gradually, and the differences between the groups were statistically significant. RT-PCR results showed that, with the increase concentrations of cisplatin, the expression of p53β, p53 and Bax mRNA in MKN45 cells were gradually increased, and the differences between the groups were statistically significant. Pearson linear correlation analysis showed that the expression of p53β and the wild-type p53, Bax were positively correlated （r=0. 976,P 〈0. 05;r =0. 985,P 〈0.05）. Conclusion In cisplatin induced MKN45 gastric cancer cells proliferation inhibition, the p53 β isoform played as a cooperater with wide-type p53 and its downstream molecule Bax, infer that p53β isoform may be played an important role in the inhibition effect of gastric cancer cell growth.

关 键 词：顺铂 胃肿瘤 细胞系 肿瘤 P53基因 基因表达 

分 类 号：R735.2[医药卫生—肿瘤]