内皮脂肪酶基因启动子多态性与腔隙性脑梗死的相关性研究  

Relationship between LIPG gene promoter polymorphism and lacunar infarction

作　　者：杜红旗[1] 沈霞[1] 牟英峰[1] 董丽果[1] 黎普刚[1] 孙亚云[1] 窦长信[1] 侯玉超[1] 拾晴[1] 张尊胜[1] 

机构地区：[1]徐州医学院附属医院神经内科,江苏徐州221004

出　　处：《海军医学杂志》2013年第3期150-154,共5页Journal of Navy Medicine

摘　　要：目的探讨中国徐州地区汉族人群内皮脂肪酶(endothelial lipase,LIPG)基因启动子rs9958947C>T单核苷酸多态性与腔隙性脑梗死的相关性。方法采用病例-对照研究的方法,选取189例腔隙性脑梗死患者为病例组,另选202例健康体检者为对照组,采用聚合酶链反应和限性片段长度多态性方法,检测2组LIPG启动子rs9958947单核苷酸多态性。结果病例组中T等位基因频率(51.59%)和CT+TT基因型频率(78.31%)明显高于对照组(分别为43.56%和66.83%);2种基因频率组间比较分别为x^2=5.041,P=0.025和x2=6.431,P=0.011,差异具有统计学意义;Logistic回归分析显示,T等位基因、男性、吸烟、高血压、高血脂、糖尿病是徐州地区汉族人群腔隙性脑梗死的独立危险因素。结论 LIPG基因启动子rs9958947单核苷酸多态性与腔隙性脑梗死发病具有相关性。Objective To investigate the relationship between LIPG （endothelial lipase） gene promoter rs9958947C 〉 T sin- gle nucleotide polymorphism and lacunar infarction in the Han population in the region of Xuzhou. Methods One hundred and eighty- nine patients with lacunar infarction and 202 controls were recruited in the case-control study. Polymerase chain reaction and restricted fragment-length polymorphism （PCR-RFLP） were used to detect the single nucleotide polymorphisms （SNP） of the rs9958947 in LIPG promoter. Results The frequencies of the T allele （51.59% ） and CT ＋ TTgenotypes （78.31% ） in the lacunar infarction group were significantly higher than those of the control group （43.56% and 66.83% ,χ2 = 5. 041, P = 0. 025; χ2 = 6.431, P = 0.011 ）, with statistical significance. Logistic regression analysis revealed that male sex, smoking, hypertension, diabetes mellitus, hyperlipidemia and the T allele of rs9958947C 〉 T polymorphism in the promoter of LIPG gene were independent risky factors for lacunar infarction a- mong the Han population in the region of Xuzhou. Conclusion Single nueleotide polymorphism of rs9958947C 〉 T in the promoter of LIPG is closely associated with lacunar infarction.

关 键 词：内皮脂肪酶 单核苷酸多态性 腔隙性脑梗死 

分 类 号：R743.33[医药卫生—神经病学与精神病学]