应用TaqMan荧光定量RT-PCR方法检测PRRSV变异株在莱芜黑猪体内动态分布  

MEASUREMENT OF PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS LOADS IN INFECTED LAIWU BLACK PIGS WITH TAQMAN FLUORESCENCE QUANTITATIVE RT-PCR

作　　者：王辉[1,2] 李井新[1,2] 孙振[1,2] 谢之景[1,2] 姜运良[1,2] 成子强[1,2] 周恩民[3] 姜世金[1,2] 

机构地区：[1]山东农业大学动物医学院,泰安271018 [2]山东省动物生物工程与疾病防治重点实验室,泰安271018 [3]西北农林科技大学动物医学院,杨凌712100

出　　处：《中国动物传染病学报》2013年第3期63-68,共6页Chinese Journal of Animal Infectious Diseases

基　　金：转基因生物新品种培育重大专项(2009ZX08009-147B)

摘　　要：为了检测猪繁殖与呼吸综合征病毒（Reproductive and respiratory syndrome virus,PRRSV）接种莱芜黑猪后病毒在体内的增殖分布规律,将15头PRRSV阴性的莱芜黑猪仔猪随机分成两组,试验组10头,对照组5头。分别于攻毒后3、7、14、21、28 d各剖杀试验组2头,对照组1头,采集脾脏、肺脏、心脏、肝脏、肾脏、扁桃体、颌下淋巴结、腹股沟淋巴结和肠系膜淋巴结等组织,并应用建立的荧光定量RT-PCR方法对各组织中病毒进行定量。结果表明,PRRSV攻毒后d 3感染组猪的各组织中均检测到低拷贝病毒核酸存在,攻毒后7~21 d,各种组织中的病毒载量维持在较高水平,而攻毒后d 28时,多数组织中的PRRSV含量明显下降。扁桃体及各种淋巴结中的病毒载量要明显高于其他脏器,显示PRRSV在感染莱芜黑猪后对淋巴组织具有组织嗜性。In order to detect distribution of reproductive and respiratory syndrome viruses（PRRSV） in infected Laiwu black pigs,10 pigs were infected with PRRSV and 5 pigs were used as non-infected controls.Two infected pigs and one uninfected pig were sacrificed at 3,7,14,21 and 28 days post infection（DAI）.Hearts,livers,spleens,lungs,kidneys,tonsils,submandibular lymph nodes,inguinal lymph nodes and mesenteric lymph nodes were collected,and viral loads were measured in fluorescence quantitative RT-PCR.The viral copies of PRRSV were detected at 3 DAI,increased to high level at 7 to 21 DAI,and decreased significantly at 28 DAI.The viral copiesof PRRSV in tonsils and lymph nodes were significantly higher than that in other tissues,which indicated a distinct lymph tissue tropism in Laiwu black pigs.

关 键 词：莱芜黑猪 猪繁殖与呼吸综合征病毒 病毒载量检测 TaqMan荧光定量RT-PCR 

分 类 号：S852.659.6[农业科学—基础兽医学]