喉鳞状细胞癌组织和血浆中肝细胞生长因子受体的表达  

Expressions of Hepatocyte Growth Factor Receptor in the Serum and Tissues in Patients with Laryngeal Squamous Cell Carcinoma

作　　者：戴文成[1] 蒋斌[1] 韩靓[1] 

机构地区：[1]南通市肿瘤医院头颈科,江苏南通226361

出　　处：《肿瘤基础与临床》2013年第3期191-194,共4页journal of basic and clinical oncology

摘　　要：目的研究肝细胞生长因子受体(c-Met)在喉鳞状细胞癌血浆及组织中的表达,探讨c-Met在喉鳞状细胞癌发生、发展中所起的作用。方法入组喉鳞状细胞癌、喉乳头状瘤患者标本,并设立正常对照组。应用酶联免疫吸附试验检测各组血浆c-Met水平;应用半定量逆转录多聚酶链式反应和免疫组织化学法分别检测各组组织中c-Met mRNA及c-Met蛋白的表达。结果喉鳞状细胞癌患者血浆中的c-Met水平明显高于喉乳头状瘤和正常人群(P均<0.05)。喉鳞状细胞癌组织中的c-Met mRNA和蛋白水平明显高于喉乳头状瘤和正常组织(P均<0.05)。结论 c-Met在喉鳞状细胞癌血浆及组织中呈高表达,可能与喉鳞状细胞癌的发生、发展有关。Objective To study the expressions of hepatocyte growth factor receptor （c-Met） in the serum and tissues in patients with laryngeal squamous cell carcinoma, and to explore the role of c-Met in the tumorigenesis and progression of laryngeal squamous cell carcinoma. Methods The patients with laryngeal squamous cell carcinoma and laryngeal papilloma were collected, and the normal control group was established. The plasma c-Met levels of all the groups were measured by enzyme-linked immunosorbent assay, the expressions of c-Met mRNA and protein were detected by using semi-quantitative reverse transcription-polymerase chain reaction and immunohistochemistry re- spectively. Results The plasma c-Met levels of patients with laryngeal squamous cell carcinomas was higher than those in the laryngeal papilloma and the normal control group （ P 〈 0.05 ）. The expressions of c-Met mRNA and protein in the laryngeal squamous cell carcinoma tissues was higher than those in the laryngeal papilloma and the normal hepatic tissues （ P 〈 0.05 ）. Conclusion The expressions of c-Met in the serum and tissues in patients with laryngeal squamous cell carcinoma are high, c-Met may be involved in the tumorigenesis and progression of laryngeal squamous cell carcinoma.

关 键 词：肝细胞生长因子受体 喉鳞状细胞癌 酶联免疫吸附试验 逆转录多聚酶链式反应 免疫组织化学 

分 类 号：R739.65[医药卫生—肿瘤] R730.23[医药卫生—临床医学]