DLC1对人卵巢癌细胞HO-8910增殖和迁移能力的影响  

Effects of DLC1 Gene Transfection on Proliferation and Migration in Human Ovarian Cancer Cell Line HO-8910

作　　者：张海玲[1] 史惠蓉[2] 

机构地区：[1]郑州大学第二附属医院妇产科,河南郑州450014 [2]郑州大学第一附属医院妇产科,河南郑州450052

出　　处：《肿瘤基础与临床》2013年第3期205-207,共3页journal of basic and clinical oncology

摘　　要：目的探讨肝癌缺失基因1(DLC1)基因对卵巢癌细胞HO-8910增殖和迁移能力的影响及其作用机制。方法脂质体介导DLC1 cDNA转染HO-8910细胞,Western blotting检测DLC1、FAK、FAK-Y925蛋白的表达,MTT法、划痕实验测定转染前后HO-8910细胞增殖及迁移能力的变化。结果 DLC1 cDNA转染成功细胞中表达DLC1蛋白,FAK蛋白表达水平无明显变化,FAK-Y925蛋白表达水平明显下调。MTT法、划痕实验结果显示,DLC1 cDNA转染后HO-8910细胞增殖和迁移能力明显降低。结论 DLC1 cDNA导入细胞后可能通过下调FAK-Y925磷酸化水平,可抑制卵巢癌细胞体外增殖和迁移能力。Objective To investigate the effects of deleted in liver cancer （ DLC1 ） gene on proliferation and migration of human ovarian cancer cell line HO-8910. Methods A recombinant plasmid pEGFP-C3-DLC1 was transfected into HO-8910 ceils by lipofectamine. The protein expressions of DLC1, FAK, FAK-Y925 before and after transfection were detected by Western blotting. The proliferation and migration abilities of HO-8910 were determined by MTr test and scratch test. Results The stable expressions of DLC1 protein were observed in HO-8910 cells af- ter transfection of DLC1 ,the protein of FAK-Y925 was significantly decreased in these cells ,but FAK protein had no changes. The proliferation and migration abilities of cells were significantly reduced ＇after transfection of DLC1. Conclusion DLC1 gene could inhibit the proliferation and migration in ovarian cancer ceils through the FAK-Y925 pathway.

关 键 词：卵巢肿瘤 肝癌缺失基因1 增殖 迁移 

分 类 号：R737.31[医药卫生—肿瘤] R730.23[医药卫生—临床医学]