拟反相液相色谱法快速测定奶茶中咖啡因含量的研究  

Rapid Determination of Caffeine in Milk Tea by Pseudo Reversed Phase Liquid Chromatography

作　　者：董丽丽[1] 于玲[1] 李敏[1] 王力川[1] 

机构地区：[1]邢台学院生物化学系,河北邢台054001

出　　处：《分析测试学报》2013年第6期742-746,共5页Journal of Instrumental Analysis

摘　　要：采用拟反相色谱法以极性硅胶柱配以高水量的流动相,测定奶茶中咖啡因的含量,以0.03 mol·L-1甲酸钠溶液(pH 2.9)-乙腈(95∶5)为流动相,5 min内可快速完成样品测定。其线性范围为0.2～1 000mg·L-1,线性系数(r)为0.999 5,检出限(S/N=3)为0.05 mg·L-1。方法的回收率为98%～103%,相对标准偏差(RSDs)为1.9%～3.6%。A rapid and sensitive method of pseudo reversed phase liquid chromatography（pseudo-RPLC） was developed for the analysis of caffeine in milk tea.The samples were separated on a bare silica column coupled with aqueous-rich mobile phase（pH 2.9,0.03 mol？L-1sodium formate-acetonitrile,95∶5）,and tested within 5 min.The calibration curve was linear in the concentration range of 0.2-1 000 mg？L-1 with correlation coefficient of 0.999 5.The recoveries at a spiked level of 20 mg？L-1 ranged from 98% to 103% with RSDs 1.9%-3.6%.

关 键 词：咖啡因 奶茶 快速测定 拟反相色谱法 

分 类 号：O657.72[理学—分析化学] O629.3[理学—化学]