RNA干扰PI3K基因对卵巢癌SKOV3细胞增殖的影响  

Effects of PI3K-gene-targeted RNA interference on proliferation of SKOV3 cells

作　　者：毕学辉[1] 胡明英[2] 

机构地区：[1]潍坊医学院,山东潍坊261000 [2]潍坊医学院附属医院产一科

出　　处：《青岛医药卫生》2013年第3期177-179,共3页Qingdao Medical Journal

基　　金：山东省教育厅科技计划项目(NO:J08LH66)

摘　　要：目的采用RNA干扰技术沉默人卵巢癌SKOV3细胞中PI3K/AKT信号转导通路中PI3Kp110α基因,观察其对SKOV3细胞增殖的影响,以期为卵巢癌的基因治疗提供可靠的理论依据。方法体外培养人卵巢癌SKOV3细胞,脂质体介导靶向P13Kp110α基因的小干扰RNA(siRNA)转染人卵巢癌SKOV3细胞,采用免疫荧光双标法观察RNA干扰48h后对PCNA蛋白、CyclinD1蛋白的影响。结果 RNA干扰组PCNA蛋白、CyclinD1蛋白阳性率较对照组与空白载体组明显减少,而对照组与空白载体组相比无明显差别。结论 RNA干扰P13Kp110α基因可下调PCNA蛋白、CyclinD1蛋白的表达,抑制细胞增殖,这种调控可能是通过PI3K/AKT信号通路及其下游靶分子进行的。以PI3Kp110α为靶点的RNA干扰技术有望成为卵巢癌基因治疗的新策略。Objective To study the high sensitivity C-reactive protein（hs-CRP） levels in the primary early-onset hypertension patients. Methods This study included 60 cases young peo- ple suffering from hypertension and 60 cases health youth as a control group. The hypertension group was diagnosed as early-onset hypertension. All observed objects were detected blood plas- ma hs-CRP, to analyze its correlation with hypertension. Results The levels of hs-CRP in hyper- tensive patients were higher than the persons whose blood tension was normal. The difference was statistically significant（P〈0.05）. Conclusion Serum hs-CRP is associated with primary ear- ly-onset hypertension.

关 键 词：RNA干扰 卵巢癌SKOV3细胞系 P13K PCNA CYCLINDL 

分 类 号：R737.31[医药卫生—肿瘤]