转录共激活子p300在人牙髓细胞中的表达及成牙本质向分化对其表达的影响  

The expression of transcription coactivator p300 in human dental pulp cells and the effect of odontogenic induction on its expression

作　　者：王彤[1] 宁艳洋[1] 张德茜[1] 徐琼[1] 

机构地区：[1]中山大学光华口腔医学院·附属口腔医院,广东省口腔医学重点实验室,广州510055

出　　处：《中华口腔医学研究杂志（电子版）》2013年第3期14-17,共4页Chinese Journal of Stomatological Research(Electronic Edition)

基　　金：国家自然科学基金(81170953);广东省自然科学基金(S2011010004931)

摘　　要：目的探讨体外培养人牙髓细胞中转录共激活子p300的表达模式,及成牙本质向分化诱导是否影响牙髓细胞中p300的表达水平。方法体外培养人牙髓细胞,实时荧光定量聚合酶链反应(PCR)和Western blot检测P0到P7代细胞中p300mRNA和蛋白的表达情况;取P3代细胞进行成牙本质向分化诱导,于第7天和第14天分别检测p300mRNA和蛋白的表达量。结果体外培养人牙髓细胞中可检测到p300表达,表达量随细胞传代逐渐减少(P<0.05);成牙本质向分化诱导7天和14天,p300表达量低于未诱导组细胞(P<0.05),诱导14天表达量低于诱导7天(P<0.05)。结论人牙髓细胞中表达p300,表达量随体外培养传代逐渐减少;成牙本质向分化诱导下调p300表达水平。Objective To investigate the expression pattern of p300 in human dental pulp cells （DPCs） in vitro and the effect of odontogenic differentiation induction on its expression. Methods DPCs were collected from extracted human teeth and cultured. The DPCs from P0 to P7 were harvested. The expressions of p300 mRNA and protein were detected by real-time qPCR and Western blot analysis. Then the DPCs were treated with induction of odontogenic differentiation for 7 days and 14 days, and the p300 mRNA and protein levels were analyzed by real-time qPCR and Western blotting. Results Real-time qPCR and Western blot analysis showed that the expressions of p300 mRNA and protein can be detected in DPCs, and p300 levels gradually decrease with continuous passage. Furthermore, p300 expression was reduced after the induction of odontoblastic differentiation for 7 days and 14 days （P 〈 0.05）. Conclusions The expression of transcription coactivator p300 is present in human DPCs, and the expression of p300 could reduce during continuous passage. The expression of p300 in DPCs decreased after induction of odontogenic differentiation.

关 键 词：转录共激活子p300 牙髓细胞 成牙本质向分化 

分 类 号：R780.2[医药卫生—口腔医学]