不同加载时间流体剪切力对成骨细胞Ⅰ型胶原合成及碱性磷酸酶活性的影响  

Effect of different loading time of fluid shear stress on type I collagen synthesis and alkaline phosphatase activities in MC3T3-E1 cells

作　　者：陈琳[1] 麦志辉[1] 彭助力[1] 张静兰[2] 艾虹[1] 

机构地区：[1]中山大学附属第三医院口腔科,广州510630 [2]广东省口腔医院儿童牙科

出　　处：《中华口腔医学研究杂志（电子版）》2013年第3期36-39,共4页Chinese Journal of Stomatological Research(Electronic Edition)

基　　金：国家自然科学基金(81070860);广东省科技计划(2011B080701070)

摘　　要：目的探讨不同加载时间流体剪切力对成骨细胞Ⅰ型胶原(COL-Ⅰ)转录及分泌的影响。方法通过自行构建的平行平板流体加载装置,对MC3T3-E1细胞施加单次12dyn/cm2流体剪切力0、0.5、1、2及4h;利用荧光定量聚合酶链反应(PCR)及免疫组化染色检测COL-Ⅰ转录及分泌情况;并检测细胞的碱性磷酸酶(ALP)活性。结果成骨细胞经流体剪切力加载12h,增加COL-Ⅰ转录;24h后,增加COL-Ⅰ的合成及分泌;48h后,提高ALP的活性;其中细胞对12dyn/cm2流体剪切力1h的加载响应最明显。结论不同加载时间的12dyn/cm2流体剪切力能够提高MC3T3-E1前体成骨细胞COL-Ⅰ的转录分泌及ALP活性,但加载1h最合适。Objectives To investigate the effect of different loading time of fluid shear stress on synthesis of type I collagen （COL- Ⅰ ） and alkaline phosphatase （ALP） activities in MC3T3-E1 cell. Methods MC3T3-EI cells were subjected to fluid shear stress （12 dyn/cm^2） using a parallel plate flow system for 0, 0.5, 1, 2 and 4 h. The mRNA and protein expression of COL-Ⅰ were detected by quantitative PCR and immunohistochemical staining, and ALP activities were examined. Results After different loading time of fluid shear stress treatment, the transcription of COL-Ⅰ was up-regulated at 12 hours, and the synthesis of COL-Ⅰ was enhanced at 24 hours. Alkaline phosphatase activities were improved at 48 hours and the optimal loading time is 1 hour. Conclusions Different loading time of 12 dyn/cm^2 fluid shear stress could promote the synthesis of COL-Ⅰ and ALP activities in MC3T3-E1 cells. The opimtal loading time is 1 hour.

关 键 词：流体剪切力 Ⅰ型胶原 碱性磷酸酶 

分 类 号：R782[医药卫生—口腔医学]