Development and Characterization of West Nile Virus Replicon Expressing Secreted Gaussia Luciferase  

作　　者：Chao Shan Xiaodan Li Chenglin Deng Baodi Shang Linlin Xu Hanqing Ye Zhiming Yuan Bo Zhang 

机构地区：[1]Center for Emerging Infectious Diseases, State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences [2]Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences

出　　处：《Virologica Sinica》2013年第3期161-166,共6页中国病毒学（英文版）

基　　金：supported by the National Natural Science Foundation of China (grant No. 31170158 and 31000090);the '100 Talents Project' of Chinese Academy of Sciences, China (grant No. Y002171YC1)

摘　　要：We developed a Gaussia luciferase (Gluc) reporter replicon of West Nile virus (WNV) and used it to quantify viral translation and RNA replication. The advantage of the Gluc replicon is that Gaussia luciferase is secreted into the culture medium from cells transfected with Gluc replicon RNA, and the medium can be assayed directly for luciferase activity. Using a known Flavivirus inhibitor (NITD008), we demonstrated that the Gluc-WNV replicon could be used for antiviral screening. The Gluc-WNV-Rep will be useful for research in antiviral drug development programs, as well as for studying viral replication and pathogenesis of WNV.We developed a Gaussia luciferase （Gluc） reporter replicon of West Nile virus （WNV） and used it to quantify viral translation and RNA replication. The advantage of the Gluc replicon is that Gaussia luciferase is secreted into the culture medium from cells transfected with Gluc replicon RNA, and the medium can be assayed directly for luciferase activity. Using a known Flavivirus inhibitor （NITD008）, we demonstrated that the Gluc-WNV replicon could be used for antiviral screening. The Gluc-WNV-Rep will be useful for research in antiviral drug development programs, as well as for studying viral replication and pathogenesis of WNV.

关 键 词：REPLICON West Nile virus High-throughput assay 

分 类 号：TQ464.8[化学工程—制药化工]