小儿脑胶质瘤p16基因的表达  

Expression of p16 gene in pediatric gliomas

作　　者：马劲光 李能德[2] 冉德森 

机构地区：[1]花都市人民医院神经外科,花都527300 [2]华西医科大学第一临床学院神经外科,成都610041

出　　处：《中国微侵袭神经外科杂志》2000年第1期32-34,共3页Chinese Journal of Minimally Invasive Neurosurgery

摘　　要：目的检测20例小儿脑胶质瘤中p16基因及其蛋白的存在情况。方法用聚合酶链式反应－单链构象多态性分析和免疫组织化学技术检测了20例1－14岁小儿脑胶质瘤。结果显示p16基因和p16蛋白的丢失率分别为50％（10／20）及（5／20）。结论本文结果提示p16基因和蛋白缺失可能与部分小儿脑胶质瘤发生、发展有关。objective To detect the exiting situation of p16 gene and protein in 20 cases of pediatric gliomas. Method 20cases of brain glioma were deteted by PCR- SSCR and immunohistochemistric methods. Result The positive rate for loss ofp16 gene and protein were 50%(10/20) and 25%(5/20) respectively. Condusion It was indirectly suggested that loss p16 geneand protein expression might account for the pathogenesis and development of some of pediatric gliomas.

关 键 词：脑胶质瘤 P16基因 儿童 免疫组织化学 PCR-SSCP 

分 类 号：R739.4[医药卫生—肿瘤]