9.4T^1 H-MRS定量分析三种乳腺癌细胞的水溶性代谢物  

Quantitative analysis on water-soluble metabolites of three kinds of breast carcinoma cells with 9.4T^1 H-MRS

作　　者：张苗苗[1] 章杰[2] 徐志峰[3] 陈耀文[4] 陈义兵[5] 吴仁华[1] 

机构地区：[1]汕头大学医学院第二附属医院影像科,广东汕头515041 [2]汕头大学精神卫生中心,广东汕头515041 [3]佛山市第一人民医院医学影像科,广东佛山528000 [4]汕头大学中心实验室,广东汕头515063 [5]中山大学肿瘤防治中心,广东广州510060

出　　处：《中国医学影像技术》2013年第6期890-893,共4页Chinese Journal of Medical Imaging Technology

基　　金：国家自然科学基金重点项目(30930027);国家自然科学基金(60971075);广东省自然科学基金(S2011010005194);汕头大学科研启动基金(NTF10010)

摘　　要：目的利用9.4T磁共振频谱仪离体检测3种乳腺癌细胞的水溶性代谢物频谱特征。方法培养获取ZR-75-1、T47-D及SK-BR-3乳腺癌细胞,用高氯酸提取水溶性代谢物,以9.4T高场磁共振仪检测所提取水溶性代谢物的氢质子频谱,定量计算谱线中相应代谢物的浓度并分析其特征。结果 ZR-75-1、T47-D及SK-BR-3乳腺癌细胞具有3种不同代谢物特征;3种乳腺癌细胞在乳酸及胆碱处均出现波峰,且T47-D的乳酸及胆碱浓度高于ZR-75-1和SK-BR-3。结论利用9.4T磁共振频谱仪能获取3种乳腺癌细胞的代谢信息;T47-D乳腺癌细胞内乳酸及胆碱代谢高于ZR-75-1和SK-BR-3。Objective To explore the features of spectrum of water soluble metabolites of 3 kinds of breast cancer ceils in vitro with 9.4T 1 H-MRS. Methods Three kinds of human breast carcinoma ceils （ZR-75-1, T47-D, SK-BR 3） were cultured and collected. The metabolites were extracted with perchloric acid and prepared for MRS analysis. ^1H spectra were acquired with 9.4T high resolution MRS, and the concentrations of main metabolites were quantitatively calculated. Results ZR-75-1, T47-D and SK-BR-3 breast cancer ceils had characteristics of three different metabolites. The metabolic peak of lactate and choline were observed in all 3 kinds of breast cancer cells. The concentration of choline-compounds and lactate in T47-D were higher than those of ZR-75-1 and SK-BR-3. Conclusion The metabolic profiles of three subtypes of human breast carcinoma cells can be reliably detected and quantified with 9.4T MRS. The metabolic of lactate and choline-compounds of T47-D cell are higher than those of ZR-75-1 and SK-BR-3.

关 键 词：磁共振波谱 乳腺肿瘤 代谢物 细胞 

分 类 号：R737.9[医药卫生—肿瘤] R445.21[医药卫生—临床医学]