5-Fu诱导人大肠癌SW480细胞株耐药与自噬活性的变化  

Relationship of the Autophagy and Resistant Induction of Colorectal Cancer Cell SW480 to 5-Fu in Vitro

作　　者：胡万乐[1] 何占红 蒋芳[1] 刘长宝[1] 

机构地区：[1]温州医学院附属第二医院中西医结合肛肠外科,温州325027 [2]深圳市光明新区人民医院,深圳518106

出　　处：《中国中西医结合外科杂志》2013年第3期279-282,共4页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：温州市2012年第一期科技计划项目(Y20120012)

摘　　要：目的:观察结直肠癌细胞产生5-Fu耐药过程中自噬水平的变化,探讨自噬活性变化在结直肠癌细胞耐药形成过程中的作用。方法:用浓度递增筛选法诱导耐受不同5-Fu浓度的耐药SW480细胞株,MDC染色和透射电镜观察SW480细胞内的自噬泡数量,CCK-8法检测细胞活力。结果:成功的诱导出三个水平的耐药细胞株;随着耐药浓度的升高,结直肠癌细胞的自噬水平逐渐增高,高水平耐药细胞株内自噬泡的数量较低水平耐药及正常SW480细胞显著增高(P<0.05),而5-Fu对其抑制率却相应的降低。结论:细胞自噬活性的增加参与了结直肠癌细胞对5-Fu的耐药过程。Objective To investigate the role of autophagy activity in the formation of durg resistance to 5-Fu, we made colorectal cancer cell SW480 resistant to 5-Fu in vitro and tried to explore the relationship be- tween autophagic levels and drug resistance. Methods Stepwise 5-Fu selection was used to establish the 5-Fu resistant subliMes. Autophagic vacuoles in cells were observed by means of transmission electron microsco- py and monodansylcadaverine （MDC）. Cell proliferation inhibition rate were measured by CCK8. Results The 5-Fu resistant cell lines we established can be resistant to 25 Ixg, 50 p^g, i00 lxg/L 5-Fu. the numbers of autoph- agic vacuoles in the 5-Fu resistant cell lines were obviously more than those in normal SW480cells（P 〈 0.05）, gradually increasing with the levels of resistance, but the level of cell proliferation inhibition rate was markedly lower in the 5-Fu resistant cell lines than those in control ceils. Conclusion 5-Fu resistant SW480 cells have greater autophagy and autophagy is involved in 5-Fu resistance.

关 键 词：结直肠癌 自噬 耐药 SW480 

分 类 号：Q95-33[生物学—动物学] R735.34[医药卫生—肿瘤]