大鼠生命早期的免疫应激对垂体促性腺激素分泌的影响  

Effects on the secretion of reproductive hormones in adult female rats exposed to lipopolysaccharide in neonatal life

作　　者：吴雪清[1] 周强勇[1] 陈海燕[1] 李晓峰[1] 李海鹰[2] 

机构地区：[1]温州医学院附属第一医院妇产科,325000 [2]温州医学院附属第一医院心内科,325000

出　　处：《中华医学杂志》2013年第21期1674-1676,共3页National Medical Journal of China

基　　金：教育部高等学校博士学科点专项科研基金新教师类联合资助课题（20113321120004）;温州市科技计划项目（2010H0051）

摘　　要：目的观察生命早期免疫应激对黄体生成素（LH）脉冲分泌的长期影响及其作用机制。方法SD大鼠在出生后第3、5天腹腔注射内毒素50μg／k（实验组）和生理盐水（对照组）。在生后12周时行双侧卵巢切除术并植入17β-雌二醇胶囊，同时行动物心导管插管，留置静脉采血装置。对其每5min采血25μl，共5h。在基础值后2h，两组动物给予静脉注射内毒素25μg/kg或者生理盐水300μl。用RT—PCR方法测定给药3h后内侧视前区（mPOA）中促肾上腺激素释放激素（CRH）及其受体（CRH-R1和CRH-R2）的基因表达。结果两组成年动物基础水平的LH分泌差异无统计学意义，而静脉注射内毒素后实验组动物的LH分泌抑制要较对照组明显的多（P〈0．05）。基础水平的CRH-R1mRNA在两组中没有区别，而静脉注射LPS后实验组的CRH-R1mRNA表达较对照组明显增强。而CRH及CRH—R2mRNA则在各组表达均差异无统计学意义。结论生命早期的免疫应激能致敏成年时期同型应激导致的对LH分泌的抑制作用，其机制可能是通过上调mPOA中的CRH—R1的基因表达所致。Objective To explore whether neonatal endotoxin challenge alters reproductive hormone secretion in adult female rats. Methods Rats were intraperitoneally injected with lipopolysaccharide （LPS） or normal saline at postnatal days 3 and 5. At Week 12, the animals underwent bilateral oophorectomy and were exposed to LPS or saline. The secretion levels of luteinizing hormone （ LH ） and estradiol were measured. Results The LPS injection in adult neonatal saline rats caused the suppressed secretion of LH and estradiol. However, the decreased secretion of both LH and estradiol were much more apparent in adult neonatal LPS-treated rats. Conclusion The neonatal LPS challenge alters the secretions of LH and estradiol during adulthood. And the mechanism may be mediated by epigenetic programming of hypothalamic- pituitary-gonadal axis.

关 键 词：免疫应激 促性腺激素 促肾上腺皮质素释放激素 

分 类 号：R[医药卫生]