一氧化氮合酶抑制剂对深低温停循环后脑再灌注损伤的保护作用  

The Effect of Nitric Oxide Synthase Inhibitor on Reperfusion Injury of the Brain under Deep Hypothermic Circulatory Arrest

作　　者：管玉龙[1] 杜月河[2] 何美玲[1] 杨璟[1] 万彩红 杜顺平[2] 董培青[1] 

机构地区：[1]首都医科大学附属北京安贞医院体外循环科,北京市100029 [2]首都医科大学附属北京安贞医院体外循环科实验外科,北京市100029 [3]太原市山西省人民医院病理科

出　　处：《中国循环杂志》2000年第4期244-245,共2页Chinese Circulation Journal

摘　　要：目的 :研究一氧化氮合酶抑制剂 (L- NAME)对深低温停循环 (DHCA)后脑再灌注损伤的保护作用。　　方法 :8只幼猪随机分为实验组 (n=4)和对照组 (n=4) ,建立体外循环 (CPB) ,行 DHCA90分后恢复灌流 12 0分 ,实验组动物静脉注射 L- NAME1.5 mg/ kg,随后在 6 0分内再给予相同的剂量。　　结果 :实验组 Na+ - K+ 三磷酸腺苷酶、超氧化物歧化酶、谷胱甘肽过氧化物酶活力明显高于对照组 ,丙二醛含量低于对照组。病理检查及电子显微镜超微结构显示实验组与对照组细胞损伤存在显著性差异 (P<0 .0 5 )。　　结论 :L- NAME对 DHCA后脑再灌注损伤有保护作用。Objective:To investigate the protective effects of nitric oxide synthase inhibitor (N G nitro L arginine methyl ester hydrochloride L NAME) on reperfusion injury of the brain under deep hypothermic circulatory arrest(DHCA). Methods:After cardiopulmonary bypass was established in 8 piglets,circulatory arrest was performed for 90 minutes followed by reperfusion 120 minutes.In the treatment group,L NAME was administered with an intravenous injection of 1 5 mg/kg at the onset of the reperfusion followed by a 60 minute continuous venous infusion of 1 5 mg/kg. Results:A significant difference was observed between the groups regarding the concentrations of ATPase,superoxide dismutase (SOD),glutathione peroxidase (GSH PX),malondialdehyde (MDA).The morphological change and ultrastructure of neuron all showed milder injury in treatment group than that in control group. Conclusion:The results suggest that L NAME protects the brain against reperfusion injury under deep hypothermic circulatory arrest.

关 键 词：深低温停循环 脑再灌注损伤 一氧化氮合酶抑制剂 

分 类 号：R743[医药卫生—神经病学与精神病学]