蕨菜试管苗生微型根茎繁殖的研究  

Propagation of Miniature Rhizome in Bracken Test-tube Plantlet

作　　者：李冬雪[1] 孙佳[1] 周清[1] 杨文新[1] 姜长阳[1] 

机构地区：[1]辽宁师范大学生命科学学院,辽宁大连116081

出　　处：《内蒙古农业科技》2013年第3期35-37,共3页Inner Mongolia Agricultural Science and Technology

基　　金：辽宁省高等教育教学改革资助项目(20090304);辽宁省大学生创新创业训练项目(201203015011)

摘　　要：为保护资源、实现栽培,试验研究以蕨菜的一年生根茎为材料,采用组织培养的方法,进行了试管苗微型根茎的培养、微型根茎段的苗床扦插、微型根茎苗的移植研究。结果证明:在1/3MS+IAA0.2mg/L+蔗糖20g/L这种培养基上进行50d的培养,能培养出具有微型根茎的试管苗;具有3个叶片的微型根茎段在温室的苗床上进行扦插繁殖是一种理想的繁殖方法;微型根茎试管苗移植成活率为99.0%,并且移植成活的微型根茎试管苗保持了蕨菜的所有植物学性状。To protect resources and realize the cultivation,with the method of tissue culture,bracken's annual rootstock were used as material to do the research as follows:the cultivation of the tube seedlings’ micro rootstock,seedbed cutting of tube seedlings’ micro rootstock segments and the transplant of the micro rootstock’s seedlings.The result showed that: Cultivate the materials on the medium 1/3MS+IAA0.2mg/L+ sugar 20g/L for 50 days,we got tube seedlings of the micro rootstock;put the micro rootstock with 3 leaves on the seedbed was an ideal method for greenhouse cutting and breeding;and the survival rate of transplanted plantlets’ micro rootstock is 99%,and the survivals of transplanted micro rootstock plantlets keep all botany characteristics of Pteridumaquilinumvar.latiusculum.

关 键 词：蕨菜 试管苗 微型根茎 

分 类 号：S567.2[农业科学—中草药栽培]