双色荧光原位杂交检测^(60)Coγ射线诱发染色体畸变的剂量效应关系  

Dose response curves for chromosome aberrations induced by ^(60)Coγ-rays and analysed by dual colour fluorescence in situ hybridization

作　　者：徐永忠[1] 郑斯英[1] 赵经涌[1] 曹建平[1] 朱巍[1] 

机构地区：[1]苏州大学放射医学系,江苏215007

出　　处：《中华放射医学与防护杂志》2000年第4期254-256,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国防科工委基金!资助项目 (Y5 5 732 6 2 )

摘　　要：目的　探索荧光原位杂交 (FISH)技术检测稳定性染色体畸变 (易位 )作为辐射生物剂量计的可行性。方法　用 1号、4号全染色体组合探针的FISH技术及常规法检测不同剂量60 Coγ射线诱发的染色体易位率和双着丝粒体率并拟合剂量效应曲线。结果　FISH技术检出的易位率、双着丝粒体率及常规法检出的双着丝粒体率与剂量间均可拟合成线性平方方程Y =c +αD + βD2 。FISH技术和常规法检出的双着丝粒体率差异无显著性 (P >0 0 5 )。对于 1号和 4号染色体 ,辐射诱发的易位率、双着丝粒体率的观察值与基于DNA含量的预期值相比 ,差异皆无显著性。结论荧光原位杂交技术能快速、准确地检测易位且有良好的量效关系 ,因此 。Objective To explore the feasibility of using fluorescence in situ hybridization (FISH) to detect stable chromosome translocation as a biological dosimeter. Methods Translocation and dicentric frequencies in peripheral blood lymphocytes induced by 60 Co γ rays at different doses were analysed by conventional staining and dual colour FISH with composite whole chromosome specific probes for human chromosomes 1 and 4,and their dose response curves were fitted. Results All dose response curves for translocation and dicentrics from FISH analysis,as well as dicentrics from conventional staining could be described by linear quadratic equations Y=c+αD+βD 2. The difference between dicentric frequencies analysed by FISH and conventional staining were not statistically significant ( P >0 05). For radiation induced translocation and dicentrics in chromosomes 1 and 4, no significant differences were found between the observed frequencies and expected frequencies based on DNA content. Conclusion Chromosome translocation can be quickly and accurately analysed by FISH and has a good dose response relationship; thus,it is hopeful to use translocation frequencies measured by FISH as a long term or accumulative biological dosimeter.

关 键 词：荧光原位杂交 染色体畸变 剂量效应 钴60γ射线 

分 类 号：R14[医药卫生—公共卫生与预防医学]