青年与老龄昆明小鼠焦虑动物模型结构维度比较  

Comparison of the construct validity of anxiety animal models in Kunming mice between young and old

作　　者：王丽娜[1] 郭小兵[2] 马金芳[1] 杜明君[1] 潘飞[1] 

机构地区：[1]新乡医学院第二附属医院,新乡453002 [2]首都医科大学附属北京安定医院

出　　处：《中华行为医学与脑科学杂志》2013年第6期514-516,共3页Chinese Journal of Behavioral Medicine and Brain Science

摘　　要：目的 比较青年与老龄昆明小鼠焦虑动物模型结构维度的不同.方法 青年与老龄雄性昆明小鼠依次进行旷场实验（open field test,OFT）、高架十字迷宫实验（elevated plus maze,EPM）和明暗箱实验（light/dark box,LDB）,实验间隔1周,摄像系统记录5 min的行为变化,实验参数包括：OFT中央区停留时间百分率、中央区水平运动百分率（Ccross％）、总水平运动（Cross）、总垂直运动（Rear）,EPM开臂区停留时间百分率（Otime％）、开臂区进入次数百分率（Oentries％）、两臂总进入次数（Entries）,LDB明箱区停留时间百分率（Ltime％）、明箱区水平运动百分率（Lcross％）、明箱区垂直运动百分率（Lrear％）、穿梭次数（Transition）、总水平运动（Cross）、总垂直运动（Rear）.结果 老龄组与青年组相比,OFT焦虑因子参数（Ctime％,t=3.12,P＜0.01；Ccross％,t=2.37,P＜0.01）、EPM焦虑因子参数（Otime％,t=1.31,P＜0.05；Oentries％,t=1.57,P＜0.05）、LDB焦虑因子参数（Ltime％,t=4.15,P＜0.01；Lcross％,t=3.28,P＜0.01；Lrear％,t=2.73,P＜0.01）均升高,而OFT运动探索因子参数（Cross,t=2.05,P＜0.01）、EPM运动探索因子参数（Entries,t=2.09,P＜0.05）、LDB运动探索因子参数（Transition,t=2.51,P＜0.01；Cross,t=3.02,P＜0.01）均降低.结论 青年与老龄昆明小鼠焦虑动物模型具有相似的结构维度,但是随着年龄增长,焦虑情绪增加,运动探索活性降低.Objective To explore the effect of different ages on behavioral dimensions of anxiety animal models in Kunming mice.Methods Young/Old,male,Kunming mice were recorded twice,for five minutes,with a one-week inter-trial interval in open field test （OFT）,elevated plus maze （EPM） and light/dark box （LDB）.The following parameters were evaluated：percentage of time spent in the OFT central area （Ctime%）,percentage of number of squares visited in the OFT central area （Ccross%）,the total number of horizontal squares in OFT （Cross）,the total number of rears in OFT （Rear）,percentage of time spent in the EPM open arms （Otime%）,percentage of number of entries into the EPM open arms （Oentries%）,the total number of entries into the EPM open and closed arms （Entries）,percentage of time exploring in the LDB light area （Ltime%）,percentage of number of squares crossing in the LDB light area （Lcross%）,percentage of rears in the LDB light area （Lrear%）,the total number of transition in LDB （Transition）,the total number of horizontal squares in LDB （Cross） and the total number of rears in LDB （Rear）.Results OFT-Ctime%,OFT-Ccross%,EPM-Otime%,EPM-Oentries%,LDB-Ltime%,LDB-Lcross% and LDB-Lrear% had significant loadings on anxiety factor; OFTCross,EPM-Entries,LDB-Transition and LDB-Cross loaded on locomotion-exploration factor.Old mice had higher anxiety emotion （Ctime%,t =3.12,P〈 0.01; Ccross%,t =2.37,P〈 0.01; Otime%,t =1.31,P〈 0.05; Oentries%,t=1.57,P〈0.05; Ltime%,t=4.15,P〈0.01 ; Lcross%,t=3.28,P〈0.01 ; Lrear%,t=2.73,P〈0.01） and lower locomotion and exploratory activity （Cross,t =2.05,P 〈 0.01 ; Entries,t =2.09,P 〈 0.05 ;Transition,t=2.51,P〈0.01; Cross,t=3.02,P〈0.01）,compared with Young.Conclusion Ages have no effect on structural dimensions of anxiety animal models.With the growth of ages,old mice have higher anxiety emotion but lower locomotion and exploratory activity.

关 键 词：年龄 焦虑 动物模型 旷场实验 高架十字迷宫实验 

分 类 号：R-332[医药卫生]