内蒙古2009年流感病原监测及甲型H1N1流感血凝素基因分析  

Analysis on pathogen and hemagglutinin genetic variation of influenza viruses in Inner Mongolia in 2009

作　　者：李昕[1] 跃华[1] 海岩[1] 雷霞[1] 董杰[1] 

机构地区：[1]内蒙古自治区疾病预防控制中心,内蒙古呼和浩特010031

出　　处：《现代预防医学》2013年第13期2552-2555,共4页Modern Preventive Medicine

摘　　要：目的探讨2009年流感流行病毒型别及亚型在内蒙古流感样患者中的流行情况及病毒型内血凝素(HA)基因变异情况。方法采集流感样病例咽拭子标本,用Real-timePCR进行样本的检验,阳性标本进行鸡胚病毒分离培养,选取代表性毒株做病毒血凝素(HA)基因测序分析。结果 Real-time PCR方法共检测到阳性标本419份,其中甲型H1N1流感182份,占阳性样本总数的43.44%。对419份阳性样本全部进行鸡胚分离培养,获得阳性毒株162株,阳性分离率为38.66%。经鉴定甲型H1N1 155株,占阳性毒株的95.68%;甲型H1N1与季节性H3N2混合2株,季节性H3N24株,B型毒株1株。对其中3株甲型H1N1毒株进行HA测序后分析发现病毒分离株与参比毒株的HA基因序列具有高度同源性。结论 2009年在内蒙古地区流行的主要是甲型H1N1病毒,兼有其他亚型出现。甲型H1N1流感与当年的流行株无大的变异发生。OBJECTIVE To explore the type and subtype distribution of influenza viruses in influenza-like patients and the hemagglutinin （HA） genetic variation of influenza A viruses in Inner Mongolia during the influenza prevalent season in 2009. METHODS Oropharyngeal swabs were collected from the influenza-like patients in the sentinel hospitals and during the out-breaks, and then identified by real-time reverse transcription-polymerase chain reaction （ RT-PCR） , positive specimens were subsequently inoculated into embryonated egg to isolate influenza viruses. HA segments of influenza A viruses were sequenced to analyze the genetic variation of HA. RESULTS 419 positive specimens were detected with real-time PCR, including 182 （43.44%） influenza A / H1N1. 162 isolates of influenza viruses were collected from 419 positive specimens, the isolation rate being 38.66%. Of these influenza viruses isolated, 155 （95.68%） were influenza A / H1N1 2009, 2 had a mix of influenza A / H1N1 and H3N2, 4 were H3N2 and 1 was influenza B. HA sequences data showed that the virus had the high homeology with reference virus. CONCLUSION Influenza A / H1N1 is main predominant virus subtype in 2009 in Inner Mongolia. It is similar with the vaccine-like virus and the isolated virus of China.

关 键 词：甲型H1N1流感病毒 血凝素 基因变异 

分 类 号：R373.13[医药卫生—病原生物学]