微囊藻毒素缓解过氧化氢胁迫下铜绿微囊藻损伤的初步研究  

MICROCYSTIN-LR INCREASES THE FITNESS OF MICROCYSTIS AERUGINOSA UNDER H_2O_2 STRESS

作　　者：丁奕[1,2] 甘南琴[1] 郑凌凌[1] 宋立荣[1] 

机构地区：[1]中国科学院水生生物研究所,淡水生态与生物技术国家重点实验室,武汉430072 [2]中国科学院大学,北京100049

出　　处：《水生生物学报》2013年第3期515-521,共7页Acta Hydrobiologica Sinica

基　　金：国家自然科学-云南联合基金(U0833604);国家自然科学基金(31070355);重点实验室优秀基金(31123001)资助

摘　　要：过氧化氢可抑制藻类生长,同时会导致微囊藻毒素(Microcystins,MCs)的释放,实验设置4个处理组探讨了外源微囊藻毒素MC-LR对H2O2胁迫下铜绿微囊藻生理生化变化的影响。结果表明:在H2O2胁迫下,微囊藻的生长和光合活性受到显著抑制,藻细胞存活率降低,ROS含量明显增加,SOD活性上升。与单独H2O2胁迫相比,加入MC-LR能增加微囊藻细胞的存活率。250μmol/L H2O2处理24h和48h后,在培养基中加入200 ng/mL MC-LR可以缓解H2O2对铜绿微囊藻光合系统PSII活性的抑制作用。当微囊藻暴露于250μmol/LH2O2环境中时,添加了MC-LR处理组藻细胞中的ROS含量明显减少(P<0.05)。在相同浓度H2O2且加入了外源MC-LR后藻细胞SOD活性下降(P<0.05)。因此,微囊藻毒素MC-LR可缓解250μmol/L H2O2引起的氧化损伤并增强微囊藻自身的生存能力。研究结果有利于阐明H2O2胁迫影响产毒蓝藻生长代谢的途径及MCs生物学意义。Hydrogen peroxide acts as an algicide and can also rapidly decomposition into oxygen and water. Becausemany bloom-forming cyanobacteria species produce and release microcystin, we used MC-LR to test whether MC-LR was involved in H2O2 induced growth and metabolic changes in Microcystis aeruginosa. The results showed that M. aeruginosa growth was significantly inhibited after exposure to 250 μmol/L and 350 μmol/L H2O2. The addition of 200 ng/mL MC-LR could increase the cell viability of M. aeruginosa under 250 μmol/L H2O2 stress. The inhibition of PSII activity after exposure to 250 μmol/L H2O2 was also reduced in the presence of MC-LR. Moreover, the addition of MC-LR decreased ROS accumulation （P〈0.05） in cells threated with 250 mmol/L H2O2 and reduced SOD activity （P〈0.05）. Hence, MC-LR could increase the fitness of M. aeruginosa under 250 μmol/L H2O2 stress. Our findings contributed to the understanding of growth and metabolism of toxic Microcystis under H2O2 stress.

关 键 词：铜绿微囊藻 微囊藻毒素 过氧化氢 活性氧 超氧化物歧化酶 

分 类 号：X171.5[环境科学与工程—环境科学]