人中性粒细胞防御素1-3的日节律性分泌  

The circadian rhythm expression of the human neutrophil peptide 1-3

作　　者：陈意[1] 陶人川[1] 林雪芳[1] 刘贞敏[1] 雍翔智[1] 李润英[1] 

机构地区：[1]广西医科大学口腔医院牙周黏膜科,南宁530021

出　　处：《国际口腔医学杂志》2013年第4期429-431,共3页International Journal of Stomatology

基　　金：国家自然科学基金资助项目(81260167)

摘　　要：目的探索人中性粒细胞防御素1-3(HNP1-3)表达水平的日节律性,为其作为生物学指标提供科学依据。方法对6名健康志愿者,连续2 d从6时至22时定点采集唾液、外周血及4颗第一恒磨牙近颊沟处的龈沟液,运用酶联免疫吸附测定(ELISA)方法检测HNP1～3的表达量。结果 6名受试者HNP1-3的表达量和变化趋势为:龈沟液的中位数为5.86μg.L-1,总体变化趋势为正弦波形,14时最高,18时最低;唾液的中位数为2.15μg.L-1,总体变化趋势为"V"字波形,早晚较高,14时达峰谷;血液的中位数为4.79μg.L-1,总体变化趋势为单峰波形,6时至10时呈上升趋势并达峰值,继而缓慢下降。结论在3种体液中,HNP1-3表达量存在差异性,其昼夜的表达呈现一定节律性。Objective To detect the human neutrophil peptide 1-3 （HNP1-3） expression in circadian rhythm. Methods Saliva, peripheral venous blood and gingival erevicular fluid （GCF） samples from mesio-buccal gingival crevice of four first molars of six healthy individuals were collected at two consecutive days from 6：00 to 22：00, and the expression of HNP1-3 was detected by enzyme-linked immunosorbent assay （ELISA） method. Results The median in GCF was 5.86 μg·L-1, the overall trend was a sinusoidal waveform, with peak at 14：00 and valley at 20：00. The median in saliva was 2.15μg·L-1, the overall trend was the ＂V＂ word waveform, higher in the morning and evening, the valley at 14：00. The median in peripheral venous blood was 4.79 μg·L-1, the overall trend was single peak waveform, reaching a peak from 6：00 to 10：00, and then declining slowly. Conclusion The expression HNP1-3 in saliva, GCF, peripheral venous blood samples existed differences. The trend from high to low was GCF, blood, saliva and showed a certain circadian rhythm.

关 键 词：日节律性 人中性粒细胞防御素 龈沟液 外周血液 

分 类 号：R78[医药卫生—口腔医学]