环磷酰胺免疫抑制大鼠的皮肤感染模型建立  

Establishment of a skin-infection model in immunosuppressive SD rats induced by cyclophosphamide

作　　者：宋刚[1] 王新[2] 柳江枫[1] 张金鹏[1] 胡天驹[1] 彭宜红[2] 

机构地区：[1]北京大学基础医学院2009级临床医学,100191 [2]北京大学基础医学院病原生物学系,100191

出　　处：《中华微生物学和免疫学杂志》2013年第6期458-461,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金国家基础科学人才培养基金资助（J1030831/J0108）

摘　　要：目的建立一个稳定有效的大鼠皮肤感染模型，用于评价外用抗感染药物的疗效。方法成年SD大鼠皮下注射环磷酰胺建立稳定的免疫功能抑制状态后，在其背部行手术造创口，连续2d涂擦耐甲氧西林金黄色葡萄球菌菌液。肉眼观察伤口动态感染情况、伤口组织病理表现及伤口痊愈时间3项指标，作为判断建立皮肤感染模型的依据。结果人工感染的实验大鼠皮肤伤口出现典型化脓性感染，其中环磷酰胺处理组比未处理组大鼠的伤口感染情况和病理组织感染指征更重、伤口恢复速度明显减慢，痊愈时间延后大于10d。结论在环磷酰胺免疫抑制SD大鼠中能成功建立皮肤外伤感染模型，可用于评价包括抗耐药菌株在内的外用抗感染药物的疗效。Objective To establish an effective and stable rat model of skin-infection for evalua- ting the therapeutic effects of topical anti-infection drugs. Methods SD rats were subcutaneously injected with cyclophosphamide to induce immunosuppression, and then surgical incisions were made on both sides of the spine. The rat model of skin-infection was established by applying methicillin-resistant Staphylococcus aureus suspension to the incisions for two consecutive days, and evaluated by analyzing infection status, pathological changes and healing time. Results The development of pyogenic infection was detected in all of the rats. Compared with the non-cyclophosphamide treated group, the cyclophosphamide treated group showed a more severe infection both from the visual inspection and the microscopic observation, moreover, its healing time was delayed more than 10 days. Conclusion The skin-infection model was successfully es- tablished in immunosuppressed rats induced by cyclophosphamide, which could be applicable to the efficacy evaluation of anti-infection drugs for external use on skin infection.

关 键 词：动物模型 耐甲氧西林金黄色葡萄球菌 皮肤感染 免疫抑制 

分 类 号：R-332[医药卫生]