二氢青蒿素联合顺铂对胃癌细胞生长的影响  

Effect of Dihydroartemisinin combined with Cisplatin on growth of human gastric cancer SGC7901 cells

作　　者：钟轩[1] 王爱军 王红钰 冯俊伟 郑宝军 施华 

机构地区：[1]开滦总医院普通外科,河北唐山063000 [2]河北省唐山市工人医院肿瘤外科,河北唐山063000

出　　处：《中国现代医学杂志》2013年第8期47-51,共5页China Journal of Modern Medicine

摘　　要：目的探讨二氢青蒿素(DHA)联合顺铂(CDDP)对人胃癌SGC7901细胞生长的影响及作用机制。方法体外培养人胃癌SGC7901细胞,分为对照组、DHA组、CDDP组和DHA+DDP联用组,MTT比色法检测细胞增殖情况;流式细胞仪检测细胞周期分布情况及凋亡的百分比;RT-PCR和Western blotting分别测定Cyclin D1、P21、Caspase-3的mRNA和蛋白的表达水平。结果 DHA和CDDP都能明显抑制SGC7901细胞增殖,DHA+CDDP组抑制强度明显高于单一药物处理组,二者具有协同抑制作用;与对照组相比,DHA和CDDP均能使细胞周期阻滞于G1期,细胞凋亡率明显增加(P<0.05),DHA+CDDP作用组停滞在G1期细胞比例及细胞凋亡率均显著高于单独DHA或CDDP处理组(P<0.05);与对照组相比,DHA或CDDP处理组Cy-clin D1 mRNA和蛋白表达下降、P21和Caspase-3 mRNA和蛋白表达上升(P<0.05),DHA+CDDP组细胞Cyclin D1 mRNA和蛋白水平显著低于单用组(P<0.05);P21和Caspase-3 mRNA和蛋白水平显著高于单用组(P<0.01)。结论 DHA与CDDP联用对胃癌细胞的抑制具有协同作用,其作用机制可能与下调Cyclin D1和上调P21、Caspase-3基因表达,阻滞细胞周期进程和促进细胞凋亡有关。[Objective] To investigate the effect of Dihydroartemisinin （DHA） combined with Cisplatin （CDDP） on the proliferation and apoptosis in human gastric cancer SGC7901 cells and the corresponding mechanism. [Methods] Cultured SGC7901 cells were divided into 4 groups： control group, DHA group, CD- DP group and DHA＋DDP group. The proliferation of cells was detected by MTr. Cell cycles and the cell apoptosis rate were obtained by flow cytometry （FCM）. Cyclin D1, P21, and Caspase-3 expression were de- tected by RT-PCR and Western blotting, respectively. [Results] DHA or CDDP inhibited the growth of SGC7901 cells. The suppression ratio of cells treated with DHA combined CDDP was higher than that of DHA or CDDP treatment alone. A strong synergism was found between DHA and CDDP in SGC7901 cells. After SGC7901 cells were treated with DHA, CDDP, or DHA＋ CDDP, the cell population of G1 and the cell apoptosis rate were increased significantly （P〈0.05）. The cell population of G1 and the cell apoptosis rate of cells treated with DHA combined CDDP were higher than that of DHA or DDP treatment alone （P 〈0.05）. DHA or CDDP reduced the expression of Cyclin D1, and induced the expression of P21and Caspase-3 （P〈 0.05）. The changes in expression of Cyclin D1, P21, and Caspase-3 in DHA combined CDDP group weremore greater than in DHA or CDDP group （P〈0.05）. [Conclusion] DHA and CDDP have a synergetic effect on the inhibition of SGC7901 cells. The effect may be related with its down-regulation of Cyclin D1, up-reg- ulation of P21and Caspase-3, as well as induction of cell cycle arrest and apoptosis.

关 键 词：二氢青蒿素 顺铂 胃癌细胞 增殖 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]