我国南方地区羊泰勒虫病的流行病学调查  

Epidemiological investigation of theileriosis in goats and sheep in southern China

作　　者：袁延庆[1] 李有全[1] 刘志杰[1] 杨吉飞[1] 陈泽[1] 关贵全[1] 罗建勋[1] 殷宏[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室,甘肃兰州730046

出　　处：《中国兽医科学》2013年第6期551-556,共6页Chinese Veterinary Science

基　　金：国家自然科学基金资助项目(31272556;31101621;31001061);国家重点基础研究发展计划(973)项目(2010CB530206);国家引进国际先进农业科学技术计划(948)项目[2012-S4(2)];甘肃省重点项目(1002NKDA035);欧盟EPIZONE(FOOD-CT-2006-016236);PIROVAC(KBBE-3-245145)

摘　　要：为研究我国南方四省羊泰勒虫病的流行情况,分别采用血涂片镜检和PCR方法对采自我国广西壮族自治区、贵州省、广东省和重庆市部分地区的180份羊血液样品进行检测,并对部分阳性样品进行测序分析。PCR结果显示,在贵州省、广东省和重庆市的样品中均有羊泰勒虫感染,其中吕氏泰勒虫阳性率分别为46.8%(29/62)、90.9%(30/33)和12.7%(7/55),绵羊泰勒虫和尤氏泰勒虫均为阴性;在广西壮族自治区样品中没有检测到羊泰勒虫感染。系统发育树分析表明,检测到的泰勒虫与我国流行的吕氏泰勒虫亲缘关系最近(部分虫株之间同源性高达100%),与水牛泰勒虫、瑟氏泰勒虫和东方泰勒虫亲缘关系较近,与羊巴贝斯虫和牛巴贝斯虫亲缘关系较远。为进一步了解我国羊泰勒虫病的流行现状及优势虫株的分布提供了重要的参考依据。To study the prevalence of ovine and caprine theileriosis in southern areas of China, a total of 180 peripheral blood samples of sheep and goats were collected from Guangxi, Guizhou, Guangdong and Chongqing and detected by microscopic examination and PCR. Theileria 18 S rRNA gene was cloned using a part of positive samples and sequenced. PCR results showed that the infection of Theileria spp. was existed in Guizhou,Guangdong and Chongqing and the positive rate of T. luwenshuni was 46. 8% （29/62）, 90.9% （30/33） and 12.7%（7/55） ,respectively,but no T. ovis and T. uilenbergi. No infection of Theileria spp. was found in Guangxi. Phylogenetic tree analysis confirmed that the relationship of the newly identified Theileria spp. was most closely related to T. luwenshuni（100%, partly）, T. buffeli, T. sergenti and T. orientalis,but more distantly related to Babesia ovis and B. boris. The results provided important evidence for understanding the prevalence of theileriosis and the distribution of predominant strains.

关 键 词：羊泰勒虫病 流行病学调查 聚合酶链反应 18S RRNA 系统发育树 

分 类 号：S852.723[农业科学—基础兽医学]