基于EST-SSRs的巴西橡胶树魏克汉种质核心种质构建研究  

Establishment of a Hevea brasiliensis Germplasm Core Collection Based on EST-SSRs

作　　者：方家林[1] 龙青姨[2] 华玉伟[1] 黄华孙[1] 

机构地区：[1]中国热带农业科学院橡胶研究所农业部橡胶树生物学与遗传资源利用重点实验室,海南儋州571737 [2]云南省热带作物科学研究所,云南景洪666100

出　　处：《热带作物学报》2013年第6期1013-1017,共5页Chinese Journal of Tropical Crops

基　　金：农作物种质资源保护专项(No.NB2013-2130135-);热带农作物种质资源保护专项(No.13RZZY-)

摘　　要：橡胶树魏克汉种质是目前橡胶树栽培品种的主要来源,主要来源于魏克汉培育46株母树繁殖的后代,亲缘关系较近。通过对289份资源EST-SSRs分析及基于EST-SSRs位点的多样性,结合种质资源来源和特殊农艺性状,构建包含27份种质的橡胶树魏克汉种质核心种质库,占原群体10%,主要来源于中国(16份)和巴西(6份)。核心种质库与原群体相比,总等位基因数保持不变,遗传多样性指数、多态信息含量、杂合度等高于原群体,基因型数目和主要等位基因频率较原群体低,较好地代表了原群体的遗传多样性。同时,核心种质农艺较为丰富,包含了高产、速生、抗寒等种质。Hevea brasiliensis clones were mostly originated from 46 clones bred with Wickham germplasm. A Wickham germplasm core collection was established based on the genetic diversity of EST-SSRs and agronomic characters. Twenty-nine resources including 16 from China and 6 from Brazil were included in the collection. Comparison of the entire collection, the corset captured 100% alleles and rare alleles of the entire collection; the values of gene diversity, heterozygosity and PIC were higher, while the values of major allele frquency and the number of allele were lower. The core Collection included rich elite clones, such as high latex yield, cold resistance, fast growth et al. clones.

关 键 词：橡胶树 魏克汉种质 EST-SSRS 核心种质 遗传多样性 

分 类 号：S794.1[农业科学—林木遗传育种]