不同品种木薯叶绿体比较蛋白质组学初步研究  

Comperative Proteomic of Chloroplast From Different Species of Manihot esculenta

作　　者：贺庭琪[1,2] 徐兵强[1,2] 郭安平[1,2] 王力敏[1,2] 王丹[2] 黄启星[2] 仝征[2] 尹奇[1,2] 王旭初[1,2] 

机构地区：[1]海南大学农学院,海南海口570228 [2]中国热带农业科学院热带生物技术研究所,海南海口571101

出　　处：《热带作物学报》2013年第6期1090-1097,共8页Chinese Journal of Tropical Crops

基　　金：国家重点基础研究发展973计划(No.2010CB126605);热带生物技术研究所基本科研业务费专项(No.ITBB110101;02);海南省自然科学基金项目(No.3100807)

摘　　要：以"华南8号"木薯(SC8)和"华南124号"木薯(SC124)的叶绿体作为研究材料,采用改进酚抽提法提取蛋白,通过单向SDS-PAGE电泳和双向SDS-PAGE电泳,比较不同木薯品种叶绿体的蛋白表达谱,并对表达的差异蛋白进行MALDI-TOF MS质谱鉴定,获得15个差异蛋白,其中有6个蛋白在SC124木薯叶绿体中表达较高,9个蛋白表达很低。对蛋白进行功能分析,发现差异蛋白主要参与蛋白翻译后修饰、周转、分子伴侣、碳水化合物运输等过程。通过RT-PCR验证了木薯核酮糖1,5-二磷酸羧化酶、ATP合酶β亚基的基因表达情况,结果表明,ATP合酶β亚基基因表达与蛋白质的表达比较一致,而核酮糖1,5-二磷酸羧化酶基因与蛋白质表达变化不一致。The chloroplast of the cassava cultivar ＇South China 8＇ （SC8） and ＇South China 124＇ （SC124） was used to extract the chloroplast protein with an improved phenol （BPP） method, and the chloroplast protein expression profiles of different cassava varieties were determined with 1-DE and 2-DE,and the differentially expressed proteins were identified with MALDI-TOF MS mass spectrometry. Six protein spots were found to be expressed highly among the 15differantially expressed protein spots of SC124. Protein functional classification analysis revealed that half of the identified proteins were involved in carbohydrate transport and posttranslational modification as molecular chaperones. RT-PCR analysis demonstrated that the ATP synthase beta subunit gene had similar expression pattern with its protein level in chloroplast, whereas ribulose 1,5-bisphosphate carboxylase gene and protein showed a different correlation with each other. The above results may be valuable for further comparison of the cassava chloroplast proteome during different developments or under different stressed conditions.

关 键 词：木薯 叶绿体 比较蛋白质组学 质谱 RT-PCR 

分 类 号：Q946.1[生物学—植物学]