全反式维甲酸增强替莫唑胺对U251细胞化疗效果的研究  

All-trans retinoic acid-induced increase in the chemotherapeutic effect of temozolomide on U251 glioma cell line

作　　者：吴长松[1] 徐国政[2] 张新元[2] 吴越[1] 王在贵[2] 高宝成[2] 

机构地区：[1]南方医科大学武汉临床学院神经外科,武汉430070 [2]广州军区武汉总医院神经外科,武汉430070

出　　处：《中国临床神经外科杂志》2013年第6期365-368,共4页Chinese Journal of Clinical Neurosurgery

摘　　要：目的观察全反式维甲酸(ATRA)对替莫唑胺(TMZ)治疗人脑胶质瘤是否有协同作用。方法用ATRA、TMZ或两药联合分别作用于人脑胶质瘤细胞株U251细胞,细胞划痕法检测各组的细胞迁移能力,流式细胞术检测细胞凋亡率,免疫荧光检测CD133细胞阳性率。结果 ATRA、TMZ均能抑制U251细胞的迁移,联合用药时迁移抑制更明显。对照组、ATRA组、TMZ组及联合用药组U251细胞凋亡率分别为(6.24±0.81)%、(24.93±4.58)%、(21.36±3.76)%和(54.27±4.83)%,CD133阳性细胞率分别为(7.05±0.27)%、(0.66±0.30)%、(36.32±3.22)%和(4.70±0.52)%。各组间细胞凋亡率和CD133阳性细胞率均有明显差异(P<0.01)。结论 ATRA和TMZ两药均可抑制U251细胞株迁移,促进细胞凋亡;两药联合应用可增强TMZ对U251细胞的化疗效果;其机制可能与ATRA减少CD133阳性细胞率有关。Objective To investigate whether there is synergic curative effect of all-trans retinoic acid （ATRA） on glioma. Methods Glioma cell line U251 cells were divided into 4 groups, i.e. ATRA treatment, temozolomide （TMZ） treatment, ATRA plus TMZ treatment and bank groups. U251 cells migration was detected by cell scratch test, U251 cells apoptosis was detected by flow cytometry, and the CD133 positive rate of U2I cells was analyzed by immunofluorescence in all the groups. Results The cell migration was significantly inhibited in ATRA treatment and TMZ treatment groups, and it was more significantly inhibited in ATRA plus TMZ treatment group compared to those in ATRA treatment and TMZ treatment groups. The U251 ceils apoptosis rates in ATRA, TMZ, and ATRA plus TMZ treatment groups and the bank group were （24.93±4.58）%, （21.36± 3.76）%, （54.27±4.83）% and （6.47±0.81）% respectively. The U251 cells CD133 positive rates in ATRA, TMZ and ATRA plus TMZ treatment groups and the bank group were （0.66± 0.30）%, （36.32±3.22）%, （4.70±0.52）% and （7.05±0.27）% respeetiely. There were significant differences in the U251 ceils apoptosis rates and CD133 positive rates among all the groups （P〈0.01）. Conclusions ATRA and TMZ may inhibit the U251 ceils migration and promote apoptosis of U251 cells. ATRA may enhance curate effect of TMZ on U251 cells. The above-mentioned synergism of TARA and TMZ may be relate to regulation of CD133 positive rate in U251 cells by ATRA.

关 键 词：全反式维甲酸 替莫唑胺 U251细胞 化疗增敏 

分 类 号：R739.41[医药卫生—肿瘤] R73-361[医药卫生—临床医学]