HIF-1α基因3′非编码区多态性与胰腺癌发生发展的相关研究  

Association of polymorphism in the 3′ untranslated region of hypoxia-inducible factor-1α gene with the susceptibility and progression of pancreatic cancer

作　　者：张圣杰[1] 王秀超[1] 贾玲玲[1] 任贺[1] 郝继辉[1] 

机构地区：[1]天津医科大学附属肿瘤医院,天津300060

出　　处：《山东医药》2013年第20期1-3,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30973490;30900596;30901448;81172355)

摘　　要：目的探讨缺氧诱导因子1α(HIF-1α)基因3′非编码区多态性与胰腺癌发生、发展的关系。方法以病例对照研究方法,采用聚合酶链扩增(PCR)和DNA序列测定分析技术,对291例胰腺癌患者(病例组)和247例健康对照者(对照组)的HIF-1α基因多态性位点进行特异扩增检测,分析HIF-1α3′非编码区SNP位点rs2057482基因型分布与胰腺癌发病风险的关系和对胰腺癌患者临床病理特征的影响。结果 HIF-1α基因rs2057482位点C/C基因型在病例组和对照组的频率分别为73.5%和61.9%,rs2057482位点C/T+T/T在病例组和对照组的频率分别为26.5%和38.1%,两者差异有统计学意义(P<0.01)。携带C/C基因型者患胰腺导管腺癌的危险性是携带纯合C/T+T/T基因型者的1.684倍(95%CI:1.242～2.314)。通过Logistic回归分析,胰腺癌中C/C基因型患者生存时间为(14.0±2.1)个月,低于C/T+T/T基因型患者的(25.2±6.3)个月(P=0.01),且该位点在是否淋巴结转移者间基因型分布差异有统计学意义(P<0.05)。结论 HIF-1α基因3'非编码区rs2057482位点的单核苷酸多态性在胰腺癌发生、发展过程中发挥作用。Objective To investigate whether the polymorphisms of hypoxia-inducible factor 1 alpha（HIF-1） in 3′ untranslated region are involved in the susceptibility and progression of pancreatic cancer（PC）.Methods The association between the genotype distribution of HIF-1 in 3′ untranslated region rs2057482 SNP and the risk of PC,and the influence on clinicopathological features of PC were analyzed in a hospital-based,case-control study,which included 291 patients with PC and 247 controls.Genotypes of HIF-1 were determined by polymerase chain reaction（PCR） and sequence analysis.Results The C/C genotype distributions of HIF-1α gene rs2057482 were 73.5% in patients with PC and 61.9% in controls,with a significant difference;the C/T＋T/T distributions were 26.5% in patients with PC and 38.1% in controls（P〈0.01）.Heterozygous genotype（C/C） of the rs2057482 polymorphism had a higher risk of developing PC,compared with homozygous genotype（C/T＋T/T）（OR=1.684,95% CI：1.242-2.314）.By performing Logistic analysis,the survival time of PC patients carrying C/C genotype（14.0±2.1 months） was significantly lower than those of C/T＋T/T genotype（25.2±6.3 months,P=0.01）.And significant difference was found in lymph node metastasis between C/T＋T/T and C/C groups（P〈0.05）.Conclusion This study suggests that the HIF-1 rs2057482 SNP in 3′ untranslated region confer susceptibility and progression to pancreatic carcinogenesis.

关 键 词：胰腺癌 缺氧诱导因子1Α 基因多态性 3′非编码区 

分 类 号：R735.9[医药卫生—肿瘤]