ID-LC-MS/MS方法对被动吸烟大鼠肺组织DNA中8-oxo-dGsn的检测  

Determination of 8-oxo-dGsn in the Lungs of Rats Exposed to Side-stream Cigarette Smoke by ID-LC-MS/MS

作　　者：陈连国[1,2] 王蒙蒙[1,2] 干伟[3] 陈晓乐[1,2] 罗顺斌[1,2] 蔡剑平[3] 胡国新[2] 

机构地区：[1]温州医学院研究生院,325035 [2]温州医学院药理教研室,325035 [3]卫生部北京医院/卫生部北京老年医学研究所/卫生部老年医学重点实验室,100730

出　　处：《医学研究杂志》2013年第6期30-33,共4页Journal of Medical Research

基　　金：国家自然科学基金资助项目(81171028)

摘　　要：目的用同位素稀释高效液相-串联质谱(ID-LC-MS/MS)方法检测大鼠肺组织中DNA氧化标志物8-羟基脱氧鸟苷(8-oxo-dGsn)含量,评价长期被动吸烟的大鼠肺组织DNA的氧化损伤程度。方法健康雄性SD(Sprague-Dawleyrats)大鼠20只,随机分为4组:被动吸烟1个月模型组(Smoke-1M组)及其对照组(Control-1M组),被动吸烟4个月模型组(Smoke-4M组)及其对照组(Control-4M组)。被动吸烟组每次烟熏1h,每天2次,每次用烟5支。被动吸烟1个月、4个月后,分别取大鼠肺组织检测其DNA中的8-oxo-dGsn含量。结果被动吸烟1个月后,大鼠肺组织DNA中8-oxo-dGsn含量高于其正常对照组(P<0.05);被动吸烟4个月后,肺组织DNA中8-oxo-dGsn含量比正常对照组增加显著(P<0.01)。结论被动吸烟1个月即可造成大鼠肺组织DNA的氧化损伤,被动吸烟大鼠肺组织中DNA氧化损伤程度随烟龄增长有积累趋势,被动吸烟是肺组织中DNA氧化损伤的重要原因。Objective To evaluate the oxidative DNA damage in the lungs of male Sprague - Dawley rats exposed to side - stream cigarette smoke, we examined the formation of 8 -oxo- 2＇ -deoxyguanosine （8 -oxo- dGsn） by isotope dilution HPLC - tandem mass spectrometry （ID - LC - MS/MS）. Methods Four groups of rats were used in the exposure protocol. These included rats exposed to smoke for 1 month （Smoke -1M group） and its control （Control- 1M group） , rats exposed to smoke for 4 months （Smoke -4M group） and its corresponding control （Control -4M group）. Rats were exposed 2 hours/day and 10 cigarettes/day. The lung tissues were obtained for measuring 8 - oxo - dGsn after exposing for 1 month and 4 months. Results The mean level of 8 - oxo - dGsn in the lung tissues from Smoke - 1 M group was higher than that of control - 1M group （ the difference was statistically significant, P 〈 0. 05） , and after another 3 months, the increase in the number of 8 - oxo - dGsn was more obvious （P 〈 0. 01 ）. Conclusion These results suggest that oxidative DNA damage is induced in lung DNA by exposing to side - stream cigarette smoke for only 1 month, and that the chronic exposure （4 months） to cigarette smoke enhance the formation of 8 - oxo - dGsn in rat lungs. This phenomenon of oxidative DNA damage in lung of rats is related to the side - stream cigarette smoke.

关 键 词：高效液相-质谱联用 大鼠 被动吸烟 氧化应激 8-oxo-dGsn 

分 类 号：R361[医药卫生—病理学]