真水狼蛛 Pirata piraticus 卵黄蛋白的研究  

Studies on vitellin of water wolf spider Pirata piraticus

作　　者：彭宇[1] 胡萃[1] 赵敬钊[2] 

机构地区：[1]浙江大学植保系,浙江杭州310029 [2]湖北大学生命科学学院,湖北武汉430062

出　　处：《浙江大学学报（农业与生命科学版）》2000年第1期69-74,共6页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：湖北省自然科学基金资助!(95 J0 6)

摘　　要：从真水狼蛛 Pirata piraticus卵中提取出了雌性特异性蛋白质——卵黄蛋白 .聚丙烯酰胺凝胶电泳和免疫扩散试验证明 ,该蛋白质为生殖期雌蛛所特有 .真水狼蛛的卵黄蛋白为糖脂结合蛋白 ,等电点为 p H6.7～ 7.5 .氨基酸组份分析表明其谷氨酸、天门冬氨酸的含量较高 ,而含硫氨基酸甲硫氨酸和半胱氨酸含量较低 .卵黄蛋白的分子量为 2 5 5 k D,其分子由 4个亚基组成 ,亚基分子量分别为 84k D,69k D,63k D和 49.8k D.在胚胎发育过程中 ,卵黄蛋白含量下降明显 .合适的温度和多种食物混合饲养有利于卵巢的生长发育和卵黄蛋白的合成 .真水狼蛛的卵黄蛋白可能是由卵巢自身合成的 .通过比较卵巢中卵黄蛋白含量和卵粒直径大小 。Vitellin was isolated and purified from the eggs of a water wolf spider, P. piraticus .The final preparation is existed only in reproducing females as judged by polyacrylamide gel electrophoresis and immunodiffusion method. The vitellin was defined as glycolipoprotein. The isoelecctric point is pH 6.7 7.5. The amino acid composition shows a high content of aspartic acid and glutamic acid, and low content of sulfur containing amino acid, methionine and cysteine. Molecular weight of the vitellin is 255 kD and the molecule is composed of four subunits with molecular weight of 84 kD, 69 kD, 63 kD and 49.8 kD, respectively. During embryonic development of the spider, the content of vitellin in embryos decreases rapidly. Suitable temperature and mixed feed will help the development of the ovary and the synthesis of the vitellin. Ovary may be the synthetic position of vitellin. The mechanisms how temperature and food affecting the reproduction of the spider are discussed by comparing the vitellin content of ovary and diameter of eggs.

关 键 词：真水狼蛛 卵黄蛋白 胚胎发育 温度 食物 

分 类 号：Q959.226[生物学—动物学] Q51