Claudin-5和CD99在胰腺实性-假乳头状肿瘤和神经内分泌肿瘤中的表达及其意义  

Evaluation of the expression and significance of Claudin-5 and CD99 in solid-pseudopapillary neoplasms and neuroendocrine tumors of pancreas

作　　者：郑洪彦[1] 石艳宏[1] 张丽芳[1] 陈英准[1] 

机构地区：[1]哈尔滨医科大学附属第二临床医学院病理科,150086

出　　处：《中华病理学杂志》2013年第6期372-375,共4页Chinese Journal of Pathology

基　　金：黑龙江省教育厅科学技术研究项目（11531131）

摘　　要：目的 研究内皮细胞间紧密连接蛋白Claudin-5和细胞间黏附分子CD99在鉴别胰腺实性-假乳头状瘤（SPN）与胰腺神经内分泌性肿瘤（P-NET）中的意义.方法 应用免疫组织化学MaxVision法检测于2003至2012年收集的37例SPN、21例P-NET组织中Claudin-5和CD99的表达情况.结果 37例SPN,Claudin-5在细胞膜表达,阳性率为100％；21例P-NET,Claudin-5均不表达；两者之间差异有统计学意义（P＜0.01）.CD99在SPN和P-NET中的表达模式不同,前者呈核旁点状聚集,后者在细胞膜表达,其表达率分别为91.9％（34/37）和61.9％（13/21）；两者之间差异有统计学意义（P＜0.01）.Claudin-5和CD99在SPN中的表达呈显著正相关（r=0.421,P=0.001）.结论 对于大体和镜下特征与P-NET相似的SPN病例,仅依靠形态学诊断十分困难.免疫组织化学联合检测Claudin-5和CD99则对鉴别诊断有重要意义.Objective To investigate the expression of endothelium tight junction protein Claudin-5 and intercellular adhesion molecule CD99 in solid-pseudopapillary neoplasms （SPN） and neuroendocrine tumors of pancreas （P-NET）, and their significance in the differential diagnoses. Methods hnmunohistochemical staining of Claudin-5 and CD99 was performed in 37 cases SPN and 21 cases of P-NET. Results Membranous Claudin-5 expression was observed in all cases of SPN but was absent in all cases of P-NET. The difference was significant （ P 〈 0. 01 ）. In SPN ,91.9% （34/37） of the cases displayed paranuclear dot-like immunoreaetivity for CD99; in contrast, 61.9% （13/21） of the cases of P-NET displayed membranous staining （ P 〈 0. 01 ）. There was a positive association between the expression of Claudin-5 and CD99 in SPN （r = 0. 421, P = 0. 001 ）. Conclusions Although the macroscopic and microscopic features of SPN are quite characteristic, they may not allow confident differentiation from P-NET in all cases, especially when these characteristics are not classical. If necessary, immunostaining for Claudin-5 and CD99 can help to differentiate between these entities.

关 键 词：胰腺肿瘤 神经内分泌瘤 紧密连接蛋白 

分 类 号：R735.9[医药卫生—肿瘤]