颅咽管瘤中RARα及PPARβ/δ表达与RA作用的相关性  

Correlation between Expression of RARα and PPARβ/δ and Effect of Retinoic Acid in Craniopharyngioma Cells

作　　者：白刚[1] 罗林[1] 王行桥 倪炜[1] 左频[1] 袁红平[1] 牛华涛[1] 范耀东[1] 

机构地区：[1]云南省肿瘤医院神经外科,云南昆明650118 [2]山东省威海卫人民医院神经外科,山东威海264200

出　　处：《昆明医科大学学报》2013年第5期29-33,共5页Journal of Kunming Medical University

基　　金：云南省科技厅专项研究基金资助项目(2008CD038)

摘　　要：目的检测颅咽管瘤中RARα及PPARβ/δ的表达,分析其表达量与RA作用效果的相关性,探讨RA靶向治疗颅咽管瘤的分子机制.方法 RT-PCR法检测31例体外培养的颅咽管瘤细胞中PPARβ/δ、RARα的表达情况,MTT法测定RA对PPARβ/δ、RARα不同表达的颅咽管瘤细胞的抑制率,分析其表达情况与RA作用是否存在相关性.结果 RT-PCR结果显示,不同颅咽管瘤细胞中PPARβ/δ、RARα表达存在差别.31例原代培养的颅咽管瘤细胞按其核受体表达不同分为PPARβ/δ>RARα、RARα>>PPARβ/δ、RARα>PPARβ/δ3组;MTT结果显示在相同RA药物作用下RARα>PPARβ/δ组的抑制率明显高于RARα>PPARβ/δ组、PPARβ/δ>RARα和对照组,差异有统计学意义(P<0.01).结论 PPARβ/δ、RARα的表达可作为评估RA治疗颅咽管瘤效果的有效指标.PPARβ/δ表达低的颅咽管瘤细胞对RA更敏感;靶向升高RARα或者靶向降低PPARβ/δ的表达都有益于颅咽管瘤的治疗.Objective To detect the expression of Retinoic acid receptor alpha （RAR α ） and peroxisome proliferator-activated receptor β/δ （PPAR β/δ） in craniopharyngioma cells, analyze the correlation between the expression and effect of retinoic acid （RA） , and investigate the molecular mechanism of RA targeted therapy for craniopharyngioma. Methods The expression of RAR α and PPAR β/δ in 31 cases of craniopharyngioma cells in vitro was quantified by reverse transcription-PCR. The inhibition rates of RA in craniopharyngioma cells with different expression levels of RAR α and PPAR β/δ were detected by using MTT assay. And the correlation between expression and effect was analyzed. Results The RT-PCR results showed that the expression of PPAR β/δand RAR α mRNA in craniopharyngioma cells was different. 31 cases of craniopharyngioma cells in primary culture were divided into three groups according the expression differences of nuclear receptor： PPAR β/δ 〉 RARα, RARet 〉 PPAR β/δ and RARα〉〉 PPARβ/δ. MTT results showed that the inhibition rate of RAR α 〉〉 PPAR β / group was significantly higher than the other groups under the same condition, with statistical significance （P 〈 0.01） . Conclusion The expression of PPAR β/δand RARα can be used as effective index to evaluate the effect of RA in craniopharyngioma. The craniopharyngioma with low-expression PPAR β/δ was more sensitive to RA.Targeting higher RAR αor targeting lower PPARβ/δ is beneficial to the treatment of craniopharyngioma.

关 键 词：过氧化物酶体增殖物活化受体 β δ核维甲酸受体 颅咽管瘤 

分 类 号：R739.41[医药卫生—肿瘤]