膀胱癌组织中白细胞介素-12B基因启动子区甲基化的变化及其意义  

Clinical Implications of Methylation of IL-12B Gene on Promoter Area in Bladder Cancer Tissues

作　　者：翟宇强[1] 石硕文[1] 谭云鹤[1] 曹志平[1] 杨平[1] 

机构地区：[1]西安市中心医院泌尿外科,陕西西安710003

出　　处：《昆明医科大学学报》2013年第5期58-60,共3页Journal of Kunming Medical University

基　　金：西安市社会发展引导计划基金资助项目(SF1201)

摘　　要：目的研究膀胱癌组织中白细胞介素-12B基因(IL-12B)启动子区甲基化的变化及其临床意义.方法应用甲基化特异性聚合酶链反应、Western-blot检测膀胱癌及癌旁组织78例,比较IL-12B基因启动子区甲基化及其蛋白质与膀胱癌发生发展的关系.结果 (1)膀胱癌组织IL-12B启动子区甲基化产物明显多于癌旁组织,差异有统计学意义(χ2=14.8,P=0.001);(2)膀胱癌组织和癌旁组织IL-12B启动子区甲基化产物的PMR水平差异有统计学意义(t=3.42,P=0.001);(3)膀胱癌组织IL-12B蛋白的表达水平显著低于癌旁组织(t=2.832,P=0.012).结论 IL-12B基因启动子区甲基化及其编码蛋白质的失活与膀胱癌的发生相关.Objective To analyze the clinical implications of methylation of the interleukin-12B gene （IL-12B） on promoter area in bladder cancer tissues. Methods The tissues of 78 cases of bladder cancer and para-carcinoma tissues were studied. The expression of methylation of IL-12B gene on promoter area was detected by polymerase chain reaction （PCR） . The protein expression of IL-12B was determined by western blot analysis. Results （1） The product of methylation of IL-12B gene on promoter area of the bladder cancer tissues was significantly higher than that of the para-carcinoma tissues （the chi-square = 14.8, P = 0.001） . （2） The PMR level of IL-12B gene in bladder cancer and para-carcinoma tissues was statistically significant （t = 3.42, P = 0.001） （3） The IL-12B protein expression level of bladder cancer tissues was significantly less than that of para-carcinoma tissues （t = 2.832, P = 0.012） . Conclusion The methylation of IL-12B gene on promoter area and the inactivation of IL-12B protein are significantly related to bladder cancer.

关 键 词：膀胱癌 白细胞介素-12B基因 聚合酶链反应 

分 类 号：R737.14[医药卫生—肿瘤]