姜黄素对肝细胞生长因子诱导的前列腺癌细胞上皮细胞间质化的逆转作用  

Effect of Curcumin on epithelial-mesenchymal transition of prostate cancer cell induced by HGF

作　　者：胡惠军[1] 陈新来[1] 潘勇权 邹伟文[1] 周文泉[1] 古彩红[1] 刘浩[2] 

机构地区：[1]广东省惠州市第三人民医院病理科,广东惠州516002 [2]中山大学药学院微生物与生化药学实验室,广东广州510006

出　　处：《现代肿瘤医学》2013年第7期1425-1429,共5页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(No.81272311;No.81071712)

摘　　要：目的:探讨姜黄素对肝细胞生长因子诱导前列腺癌DU145细胞上皮细胞间质化的影响及可能机制。方法:将DU145细胞分为3组:正常对照组、HGF组、HGF联合姜黄素组。分组干预48小时后,显微镜观察细胞形态变化,划痕试验及Transwell试验检测细胞迁移、侵袭能力,Western blot检测上皮细胞表面标志E-Cadherin和间质细胞表面标志Vimentin的变化,以及HGF/c-Met信号通路分子的表达情况。Real time RT-PCR检测c-Met mRNA的表达变化。结果:分组处理48h,HGF处理组细胞较正常对照组细胞迁移能力显著增强,HGF联合姜黄素处理组细胞迁移能力与HGF处理组相比明显下降;Western blot结果发现,相对于正常对照组,HGF处理组E-cadherin表达水平下降而Vimentin表达水平上升,而姜黄素能明显逆转HGF诱导的EMT相关蛋白表达的改变。进一步研究发现姜黄素能显著下调c-Met mRNA和蛋白水平,进而抑制HGF/c-Met信号下游p-AKT和p-ERK信号以及Snail的表达。结论:姜黄素可逆转HGF诱导的前列腺癌细胞DU145迁移能力,可能与其抑制c-Met的表达和上皮细胞间质化有关。Objective：To investigate the effect of Curcumin on epithelial-mesenchymal transition in prostate cancer cell line DU145 induced by HGF.Methods：DU145 cells were divided to 3 groups： normal control group,HGF treated group,and HGF ＋ Curcumin treated group.After 48h of treatment,migration of DU145 cells were detected by wound healing and transwell assay.The protein expression of E-cadherin,Vimentin,Snail,c-Met,p-AKT,and p-ERK were determined by Western blot.The mRNA expression of c-Met was determined by Real-time RT-PCR.Results：The migration of DU145 cells induced by HGF was significantly inhibited by Curcumin.Western blot showed that E-cadherin was decreased,whereas Vimentin was increased in HGF treated cells.And these effects were abolished by Curcumin.Meanwhile,Curcumin significantly inhibited c-Met expression,and then inhibited AKT and ERK signaling.Conclusion：Curcumin revised the migration of DU145 cells induced by HGF through inhibiting c-Met expression,and then inhibited the epithelial-mesenchymal transition of DU145 cell.

关 键 词：前列腺癌 姜黄素 肝细胞生长因子 上皮细胞间质化 

分 类 号：R730.2[医药卫生—肿瘤] R737.25[医药卫生—临床医学]