去氢骆驼蓬碱衍生物DH-332对人结肠癌细胞株HCT116体外侵袭能力的影响  

Effect of harmine derivative DH-332 on the invasive ability of the human colon cancer cell HCT116 in vitro

作　　者：宋婧[1,2] 周佳[1,2] 平锋锋[1,2] 阿布卡德[1,2] 尚靖[1,2] 

机构地区：[1]中国药科大学新药筛选中心,江苏南京210009 [2]中国药科大学天然药物国家重点实验室,江苏南京210009

出　　处：《现代肿瘤医学》2013年第7期1440-1443,共4页Journal of Modern Oncology

基　　金："十二五"国家重大科技专项(编号:2011ZX09401-007);"十二五"国家支撑计划项目(编号:2012BAI30B00)

摘　　要：目的:探讨去氢骆驼蓬碱衍生物DH-332对人结肠癌细胞株HCT116体外侵袭能力的影响。方法:不同浓度去氢骆驼蓬碱衍生物DH-332作用于人结肠癌细胞株HCT116后,采用细胞划痕,细胞黏附,Tran-swell侵袭实验检测去氢骆驼蓬碱衍生物DH-332对HCT116细胞体外迁移和侵袭力的影响。结果:不同浓度去氢骆驼蓬碱衍生物DH-332作用于HCT116细胞后,细胞划痕实验显示细胞迁移能力较对照组低;细胞黏附实验和Transwell侵袭实验显示DH-332作用后,细胞的黏附,侵袭能力降低,并呈时间和剂量依赖性(P<0.05)。结论:去氢骆驼蓬碱的衍生物DH-332可以抑制人结肠癌细胞HCT116体外侵袭能力。Objective：To investigate the effect on the invasive of human colon cancer cell line HCT116 induced by harmine derivative DH-332 in vitro.Methods：Human colon cancer cells HCT116 were treated with different concentrations of DH-332,and the migration and invasion ability of HCT116 in vitro were determined by scratch-wound assay,cell adhesion assay,and Transwell invasion assay.Results：After treatment with different doses of DH-332 in HCT116 cells,scratch-wound assay shows cell migration ability was lower than the control group,cell adhesion assay and Transwell invasion assay showed that the ability of adhesion and invasion descended with dose and time dependent.（P0.05）.Conclusion：Harmine derivative DH-332 can inhibit the invasion ability of human colon cancer cells HCT116 in vitro.

关 键 词：DH-332 结肠癌 迁移 侵袭 黏附 

分 类 号：R73-361[医药卫生—肿瘤] R735.35[医药卫生—临床医学]