BIRC5在非小细胞肺癌中的表达及与Tp53的关系  

Expression levels of the apoptotic inhibition molecules BIRC5 in non-small cell lung cancer and its correlation with Tp53 expression

作　　者：梁红玲[1] 黄健清[1] 李洪胜[1] 张绪超[2] 谢至[2] 

机构地区：[1]广州医学院附属肿瘤医院,广东广州510095 [2]广东省人民医院肺癌研究所,广东广州510080

出　　处：《现代肿瘤医学》2013年第7期1496-1499,共4页Journal of Modern Oncology

基　　金：广东省科技计划项目(No:2012B031800270);广州医学院博士;留学回国人员基金项目(No:2012C13)

摘　　要：目的:探讨凋亡信号分子BIRC5在肺癌与癌旁组织中的表达差异,并分析BIRC5与Tp53和临床病理资料之间的关系。方法:制备非小细胞肺癌患者的癌与癌旁组织石蜡标本的组织芯片。采用免疫组织化学方法检测分析BIRC5、Tp53凋亡分子在组织中的蛋白表达水平。结果:BIRC5表达水平在癌组织中(n=139)较癌旁组织(n=25)显著升高(P<0.01);BIRC5在男性患者表达水平高于女性患者,吸烟患者中表达高于非吸烟患者,鳞癌中表达高于非鳞癌(P<0.001)。BIRC5与Tp53表达水平之间存在负相关关系(r=-0.311,P=0.011)。且非参数Mann-Whiteney U检验分析显示BIRC5在Tp53高表达患者中表达高于Tp53低表达患者(P<0.05)。在I-IV期或III-IV期肺癌患者中,BIRC5高表达与低表达患者的OS均无统计学差异。结论:肺癌组织较癌旁组织BIRC5表达上调且与Tp53呈负相关关系。Objective：To investigate the expression level of the apoptotic inhibition molecule BIRC5 in non-small cell lung cancer（NSCLC） and adjacent non-cancerous tissues and its correlation with Tp53 expression and clinicopathologic parameters.Methods：Cancer versus adjacent non-cancerous tissues,formalin-fixed paraffin-embedded,of NSCLC were collected and made into tissue microarray（TMA）.Immunohistochemistry（IHC） was performed to analyze the expression levels of BIRC5 and Tp53.Results：In the analysis of non-paired 139 cases of cancers versus 25 adjacent tissues,non-parametric rank test showed that BIRC5 were expressed in cancers at a higher level than that in adjacent tissues（P0.01）.Expression levels of BIRC5 were higher in male than in female patients,smokers than non-smokers patients,and squamous than non-squamous tumors（P0.001）.BIRC5 and Tp53 expressions had negative correlation（r=-0.311,P=0.011）;Mann-Whiteney test showed BIRC5 expressed higher in patients with low levels of Tp53 than in patients with high levels of Tp53（P0.05）.In NSCLC patients with either stages I-IV or stages III-IV,there was no significant difference of overall survival（OS） in patients with high levels of BIRC5 versus low levels of BIRC5.Conclusion：BIRC5 was significantly up-regulated at a higher expression level in lung cancer tissues than adjacent non-cancerous tissues and had negative correlation with Tp53 expression.The prognostic role of BIRC5 expression and its regulatory mechanisms by Tp53 warrant further investigation.

关 键 词：肺癌 BIRC5 TP53 凋亡 

分 类 号：R734.2[医药卫生—肿瘤]