Survivin分子信标诊断膀胱肿瘤的临床应用研究  

Clinical study on diagnostic utility of Survivin-molecular beacons in bladder cancer

作　　者：王新阳[1] 贺大林[1] 杨小杰[2] 赵军[2] 

机构地区：[1]西安交通大学第一附属医院泌尿外科,陕西西安710061 [2]西安交通大学第二附属医院泌尿外科,陕西西安710004

出　　处：《现代肿瘤医学》2013年第7期1533-1537,共5页Journal of Modern Oncology

基　　金：国家自然科学基金面上项目(编号:30672102)

摘　　要：目的:探讨分子信标检测尿脱落细胞Survivn mRNA的可行性,寻找一种能够早期诊断膀胱肿瘤的方法。方法:分子信标检测膀胱肿瘤5637、J82细胞Survivin mRNA的表达,并通过Western bolt方法验证,并对35例膀胱移行细胞癌患者和35名正常健康成人行分子信标检测尿脱落细胞,Western bolt检测组织中的Survivin含量,同时行尿脱落细胞学检查。结果:Survivin分子信标检测肿瘤细胞内的Survivin表达且具有高特异性。以随机100个细胞中60个以上的细胞为阳性做为阳性标准,确定MB-cy3的阳性率为80%(28/35),特异性为77.1%(27/35);Western bolt检测的阳性率为71.4%(25/35)。两种实验方法对细胞和蛋白质中Survivin的检测结果具有一致性。尿脱落细胞学的敏感性为28.6%(10/35),特异性为100.00%(35/35)。结论:使用分子信标检测尿液脱落细胞中Survivin mRNA具有可行性,分子信标有可能成为在膀胱肿瘤的早期临床诊断和术后随访的有效工具。Objective：To develop a sensitive method for detection of bladder cancer cells the cast-off cells of bladder cancer patients,we examine the feasibility by using molecular beacon（MB） probes specific for a tumor specific Survivin mRNA.Methods：MB analyzed the survivn mRNA in bladder cancer cell 5637,J82 and identified by Western blot.Urine cytology,MB test,and Western blot were carried to test,cancer tissue of 35 bladder cancer patients and 35 health adults.Results：Survivin MB could detect the expression of Survivin gene and generated fluorescent signals in the cancer cells,and not detect in normal prostate fibroblast cells.MB detected cancerous cells in 80% of confirmed bladder cancer patients（28/35）.The specificity was 77.1%（27/35）.Survivin protein was detected by western blot in 71.4%（25/35） of these patients.The two methods had high consistency.The sensitivity and specificity of urine cytology was 28.6%（10/35）,100%（35/35）.Conclusion：Survivin MB is specific and sensitive molecular probe for detecting bladder cancer cells and urine cast-off cells of bladder cancer patients.It has great potential for the development of a clinical diagnostic procedure for early detection of bladder cancer and follow-up after operation.

关 键 词：膀胱肿瘤 诊断 分子信标 SURVIVIN 

分 类 号：R737.14[医药卫生—肿瘤]