云芝糖肽对乳腺癌患者外周血单个核细胞Toll样受体4的作用  

Effect of polysaccharopeptide on Toll-like receptor 4 in peripheral blood mononuclear cells of patients with breast cancer

作　　者：王静[1] 余爽[1] 陈楚[1] 董冰[1] 鲍依稀[1] 

机构地区：[1]重庆医科大学附属第二医院检验科,重庆400010

出　　处：《中国生物制品学杂志》2013年第6期844-847,共4页Chinese Journal of Biologicals

基　　金：国家自然科学基金(81274144);重庆市科委课题项目(CSCT;2009BB5399);重庆市教委课题项目(KJ110303);重庆市卫生局中医药科技项目(2010-2-147)资助

摘　　要：目的探讨云芝糖肽(polysaccharopeptide,PSP)对乳腺癌患者外周血单个核细胞(peripheral blood mononuclearcell,PBMC)Toll样受体4(Toll-like receptor 4,TLR4)的作用。方法体外分离乳腺癌患者或健康人PBMC,并将其分别分为PSP组(分别加入终浓度为25μg/ml的PSP和终浓度为100μg/ml的PHA)和对照组(只加入终浓度为100μg/ml的PHA),采用TLR4单克隆抗体对各组细胞进行免疫荧光染色。将乳腺癌患者PBMC分为空白对照组、TLR4抗体组、PSP组和PSP+TLR4抗体组,采用Q-PCR检测各组PBMC IL-12、IL-6和TNF-α的表达水平。结果健康志愿者对照组PBMC荧光强度较强,而健康志愿者PSP组PBMC荧光强度较弱;乳腺癌患者PSP组与乳腺癌患者对照组相比,细胞荧光强度更弱。与空白对照组比较,TLR4抗体组PBMC IL-12和组TNF-α的表达水平均显著降低(P<0.05),PSP组PBMC IL-12、IL-6和TNF-α的表达水平均显著上调(P<0.05或<0.01);与PSP和TLR4抗体组比较,PSP+TLR4抗体组PBMC IL-12、IL-6和TNF-α的表达水平均显著上调(P<0.05或<0.01)。结论 TLR4可能是PSP的作用受体之一。Objective To investigate the effect of polysaccharopeptide（PSP）on Toll-like receptor 4（TLR4）in peripheral blood mononuclear cells（PBMCs）of patients with breast cancer.Methods PBMCs were isolated from the patients with breast and healthy individuals,and divided into PSP and control groups separately.The PBMCs in PSP group were treated with PSP and PHA at final concentrations of 25 and 100 μg/ml respectively,while those in control group with PHA at a final concentration of 100 μg/ml.Cellular immunofluorescence staining was applied to PBMCs in two groups with anti-TLR4 monoclonal antibody.The PBMCs from patients with breast cancer were divided into blank control,TLR4 antibody,PSP and PSP＋TLR4 antibody groups,and determined for expression levels of IL-12,IL-6 and TNF-α by realtime quantitative PCR（Q-PCR）.Results Enhanced fluorescence intensity was observed in PBMCs from healthy individuals in control group,while that in PSP group was weak.However,the fluorescence intensity of PBMCs from patients with breast cancer in PSP group was weaker than that in control group.As compared with those in blank control group,the ex pression levels of IL-12 and TNF-α in PBMCs of patients in TLR4 antibody group decreasd significantly（P ＆lt; 0.05）,while those of IL-12,IL-6 and TNF-α in PSP group increasd significantly（P ＆lt; 0.05 or P ＆lt; 0.01）.However,compared with those in PSP and TLR4 antibody groups,the expression levels of IL-12,IL-6 and TNF-α in PSP＋TLR4 antibody groups increased significantly（P ＆lt; 0.05 or P ＆lt; 0.01）.Conclusion TLR4 may be one of the immune receptors of PSP.

关 键 词：云芝糖肽 乳腺癌 外周血单个核细胞 TOLL样受体4 免疫调节 

分 类 号：R737.9[医药卫生—肿瘤]