没药甾酮对人肝癌细胞HepG2增殖和凋亡的影响  

Effects of guggulsterone on proliferation and apoptosis of HepG2 human hepatocellular carcinoma cells

作　　者：贾晓黎[1] 石娟娟[1] 封婷[1] 程延安[1] 王蕤[1] 刘静坤[1] 党双锁[1] 

机构地区：[1]西安交通大学医学院第二附属医院感染科,西安710004

出　　处：《临床肝胆病杂志》2013年第6期452-455,共4页Journal of Clinical Hepatology

基　　金：陕西省科技攻关计划项目(2007K16-07(9))

摘　　要：目的研究没药甾酮对人肝癌细胞HepG2增殖和凋亡的影响。方法以正常人肝细胞L-02作为对照,采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐法观察不同浓度没药甾酮(5～100μmol/L)对人肝癌细胞HepG2和L-02细胞增殖的影响并观察细胞形态的变化;应用流式细胞术检测细胞周期变化和凋亡发生。结果不同浓度没药甾酮均可显著抑制人肝癌细胞HepG2生长,并呈时间、剂量依赖性,最大抑制率可达81.9%±1.92%(100μmol/L);没药甾酮可使G0/G1期细胞比例增多,G2/M期细胞比例下降,可将细胞阻滞于G0/G1期;没药甾酮诱导人肝癌细胞HepG2发生凋亡,50μmol/L和75μmol/L没药甾酮早期细胞凋亡率分别为24.91%±2.41%、53.03%±2.28%,与对照组相比,差异均具有统计学意义(P<0.05)。结论没药甾酮可抑制人肝癌细胞HepG2增殖并诱导凋亡,其作用可能与干扰细胞周期有关。Objective To investigate the potential therapeutic effects of the plant - derived polyphenol, guggulsterone, on cell proliferation and apoptosis of hepatocellular carcinoma （HCC） by using an in vitro system with the human hepatoma cell line, HepG2. Methods HepG2 cells and normal human liver L -02 cells were treated with different concentrations of guggulsterone （5 - 100 μmol/L） for 24 -72 hours. Differential effects on cell proliferation were tested by MTT assay and on cell cycle and apoptosis were detected by flow cytometry （FACS）. Results Guggulsterone significantly inhibited HepG2 cell proliferation in a dose - and time - dependent manner, with the maximal inhibition effect being observed with 100 μmol/L guggulsterone （ 81.9% ±1.92% ）. FACS analysis indicated that guggulsterone - treated tlepG2 cells were arrested in the G0/G1 phase. Guggulsterone also induced apoptosis of HepG2 ceils （ apoptotic % ： 50μmol/L, 24.91 ± 2.41 and 75μmol/L, 53.03 ± 2.28）. Conclusion Guggulsterone exerts anticancer effects on HepG2 hepatoma ceils by inhibiting cell proliferation and inducing apoptosis. The anti - proliferative effect may be related to interference of the cell cycle.

关 键 词：没药属 癌 肝细胞 细胞增殖 细胞凋亡 细胞周期 

分 类 号：R735.7[医药卫生—肿瘤]