非酒精性脂肪肝患者脂联素基因-4522位点的单核苷酸多态性  

The -4522 C/G single nucleotide polymorphism of the adiponectin gene in patients with nonalcoholic fatty liver disease

作　　者：张炜[1] 朱丽群[1] 蒋小猛[1] 曹亮[1] 程兆明[1] 徐岷[1] 

机构地区：[1]江苏大学附属医院消化科,江苏省镇江市212001

出　　处：《世界华人消化杂志》2013年第17期1660-1663,共4页World Chinese Journal of Digestology

基　　金：江苏大学临床医学科技发展基金资助项目;No.JLY20080001~~

摘　　要：目的:探讨脂联素基因-4522位点单核苷酸多态性与非酒精性脂肪肝(non-alcoholic fatty liver disease,NAFLD)的关系.方法:将140例NAFLD患者按口服葡萄糖耐量试验(oral glucose tolerance test,OGTT)进一步分为糖代谢正常组和异常组,正常健康对照组100例.检测血清脂联素,并且采用PCR-RFLP技术对脂联素基因-4522C/T进行基因分型.结果:正常人群C/C、C/T、T/T基因型比例为34.00%、52.00%、14.00%,T等位基因频率40.00%;糖耐量正常组C/C、C/T、T/T基因型比例为19.36%、50.00%、30.64%,T等位基因频率55.65%.与正常人群对比差异有显著性;糖耐量异常组C/C、C/T、T/T基因型比例为12.86%、39.29%、47.86%,T等位基因频率76.92%.与糖耐量正常组对比差异有显著性.结论:脂联素基因-4522位点存在单核苷酸多态性,NAFLD患者T等位基因频率高,T/T基因型者更易发生糖耐量异常.AIM： To detect the relationship between the -4522 C/G single nucleotide polymorphism of the adiponectin gene and nonalcoholic fatty liver disease （NAFLD）.METHODS： In total, 140 patients with NAFLD and 100 normal controls were included in the study. Patients with NAFLD were further divided into a normal glucose metabolism group and a disordered glucose metabolism group based on the results of oral glucose tolerance test （OGTT）. The adiponectin gene -4522 C/G singlenucleotide polymorphism and plasma adiponectin levels were measured in all subjects. RESULTS： The C/C, C/T and T/T genotypes were found in 19.36%, 50.00% and 30.64% of NAFLD patients with normal glucose metabolism, and in 34.00%, 52.00% and 14.00% of controls, respectively. The frequency of T/T geno-type in NAFLD patients with normal glucose metabolism was significantly higher than that in controls. The C/C, C/T and T/T genotypes were found in 12.86%, 39.29% and 47.86% of NAFLD patients with disordered glucose metabolism, respectively. The frequency of T/T genotype in NAFLD patients with normal glucose metabo-lism was significantly lower than that in patients with disordered glucose metabolism. CONCLUSION： The -4522 C/G single nucleotide polymorphism of the adiponectin gene may play an important role in the development of NAFLD. The T allelic frequency is high in NAFLD patients with disordered glucose metabolism.

关 键 词：非酒精性脂肪肝 脂联素 单核苷酸多态性 糖代谢异常 

分 类 号：R575.5[医药卫生—消化系统]