凋亡调节因子Smac、Survivin在结肠腺癌中的表达及临床意义  

Clinical significance of expression of Smac and Survivin in colon adenocarcinoma

作　　者：宋国华[1] 李维山[1] 高凤兰[1] 

机构地区：[1]漯河医学高等专科学校,河南省漯河市462000

出　　处：《世界华人消化杂志》2013年第17期1676-1680,共5页World Chinese Journal of Digestology

基　　金：河南省科技厅重点攻关基金资助项目;No.092102310234~~

摘　　要：目的:探讨第二个线粒体来源的半胱氨酸酶的激活剂(the second mitochodria-derived activator of caspase,Smac)和生存素(Survivin)在结肠腺癌中的表达及其与临床参数之间的关系.方法:采用组织芯片技术及免疫组织化学SP两步法检测59例经病理确诊的结肠腺癌组织及10例癌旁正常组织中Smac蛋白和Survivin蛋白的表达.结果:(1)Smac蛋白在结肠腺癌、癌旁正常组织中的表达率分别为66.10%(39/59),100%(10/10),差异具有统计学意义(P=0.03).Survivin蛋白在结肠腺癌、癌旁正常组织中的表达率分别为69.49%(41/59),20.0%(2/10),差异具有统计学意义(P=0.01);(2)Smac蛋白和Survivin蛋白在结肠腺癌组织中的表达与患者结肠腺癌的分化程度及淋巴结转移有关(P<0.05),但与患者的年龄、肿瘤的大小、浸润深度均无明显相关性(P>0.05);(3)Smac蛋白表达阳性的39例中,Survivin蛋白阳性表达23例,占58.97%(23/39),Smac蛋白表达阴性的20例中,Survivin蛋白阳性表达18例,占90.0%(18/20),经统计学分析,在结肠腺癌中,两者的表达明显负相关(r=-0.319,P=0.014).结论:在结肠腺癌中,Smac蛋白低表达,Survivin蛋白高表达,两者明显负相关;Smac蛋白和Survivin蛋白的异常表达可能对结肠腺癌的发生发展及淋巴结转移起着至关重要的作用.AIM： To investigate the expression of second mitochondria-derived activator of caspase （Smac） and inhibitor of apoptosis protein Survivin in adenocarcinoma of the colon and to analyze their clinical significance. METHODS： The expression of Smac and Survivin was detected by immunohistochemistry on a tissue chip containing 10 normal colon mucosal specimens and 59 colon adenocarcinoma specimens. RESULTS： The positive rate of Smac expression in colon adenocarcinoma was significantly lower than that in normal colon mucosal tissue （66.10% vs 100%, P = 0.03）, but the positive rate , of Survivin expression was significantly higher in colon adenocarcinoma than in normal colon mucosal tissue （69.49% vs 20%, P = 0.001）. Expression of Smac and Survivin in colon adenocarcinoma was both correlated with pathological grade and lymph node metastasis （both P 0.05）, but not with tumor size, infiltration depth, or patient age （all P 0.05）. The expression of Smac had a significant negative correlation with that of Survivin in colon adenocarcinoma. CONCLUSION： Low expression of Smac and high expression of Survivin closely correlate with lymph node metastasis and tumor patho-logical grade in colon adenocarcinoma. Expression of Smac positively correlates with that of Survivin in colon adenocarcinoma.

关 键 词：结肠肿瘤 免疫组织化学 SMAC蛋白 SURVIVIN蛋白 组织芯片 

分 类 号：R735.35[医药卫生—肿瘤]